急性胰腺炎大鼠内皮素-3基因表达的逆转录检测  

Evaluated on Expression of Endothelin-3 mRNA Detected by RT-PCR in Acute Pancreatitis Rats

作　　者：张勇[1] 郭永章[2] 李立[3] 曹西南[4] 

机构地区：[1]湖南省常德市第一人民医院外科,常德415000 [2]昆明医学院第二附属医院外科,昆明650041 [3]昆明市第一人民医院外科,昆明650051 [4]昆明医学院分子生物学实验室,昆明650031

出　　处：《中国普外基础与临床杂志》2007年第6期665-669,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究内皮素-3(endothelin-3,ET-3)mRNA的表达水平与急性胰腺炎时胰腺炎症程度的关系。方法将54只SD大鼠编号后随机分为4组,其中假手术组9只,胰腺炎组、动脉给药组和静脉给药组〔多巴胺:5μg/(kg.min)〕各15只,按Aho法用4.5%牛磺胆酸钠胆胰管逆行注射制作急性胰腺炎模型,分别在1、6及24h取胰腺组织,采用半定量逆转录聚合酶链式反应检测ET-3基因的转录水平,并观察各组胰腺组织病理变化,确定ET-3mRNA的表达水平与胰腺炎炎症程度的关系。结果模型制作后1、6及24h均可见到ET-3mRNA表达,各组(除假手术组外)均以6h时ET-3mRNA表达最强。除1h外,动脉给药组ET-3mRNA表达量明显低于静脉给药组及胰腺炎组(P<0.05,P<0.01);而假手术组在任何时相均明显低于另外3组(P<0.05,P<0.01)。各组胰腺组织病理学评分按假手术组→动脉给药组→静脉给药组→胰腺炎组的顺序逐渐升高。结论ET-3mRNA的表达与胰腺炎炎症程度有一定关系,ET-3mRNA表达量随胰腺炎严重程度的加重而增加;急性胰腺炎中,多巴胺动脉给药比静脉给药有效。Objective To investigate the relationship between gene expression of endothelin 3 （ET-3） and inflammation of acute pancreatitis （AP） in rats. Methods Fifty-four rats were divided randomly into 4 groups： sham operation group, AP group, arterial injection group and vein injection group. AP was induced by reverse intra bile duct infusion 4.5 % sodium taurocholate, treated with low dose dopamine [5 μg/（kg · min）] by injecting arterial or tail vein. Rats were sacrificed at 1, 6 and 24 h after the induction of AP. The mRNA expression of ET-3 was evalu- ated by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and pathological changes was observed in rats. Results Expression of ET-3 mRNA could be detected from 1 up to 24 h after the induction of pan creatitis. Expression of ET-3 mRNA of sham operation group was decreased significantly compared with other three groups. Expression of ET-3 mRNA showed a significant decrease by arterial injection dopamine than that by tail vein （P〈0.05, P〈0.01）. The pathologic score in AP group was the highest, vein injection group was the next one, and score in sham operation group was the lowest. Conclusion There are significant relationship between inflammation of AP and expression of ET-3 mRNA. Dopamine administration by arterial injection is more effective than that by tail vein injection.

关 键 词：内皮素-3 MRNA 胰腺炎 微循环 

分 类 号：R576[医药卫生—消化系统]