高压氧和低压缺氧预处理对家兔大脑中动脉闭塞后脑梗死的防治作用及其机制  

Preventive and therapeutic effects of hyperbaric oxygen and hypobaric hypoxia preconditioning on cerebral infarction after permanent middle cerebral artery occlusion in rabbits and its mechanism

作　　者：邓聪颖[1] 张礼均[1] 李飞[1] 陈志[1] 吴南[1] 王宪荣[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科

出　　处：《第四军医大学学报》2007年第23期2113-2115,共3页Journal of the Fourth Military Medical University

基　　金：国家"十一五"科技支撑计划(2006BAI01A13);全军医学科学技术研究"十一五"指令性课题(06Z037;06MB241)

摘　　要：目的:观察高压氧预处理(HBOP)和低压缺氧预处理(HHP)对兔大脑中动脉闭塞(MCAO)后局灶性脑梗死程度及周围脑组织氧分压(PbtO2)的影响.方法:雄性家兔72只随机分为单纯MCAO组,HBOP+MCAO组(100%O2,2.5ATA,1h/d,5d)和HHP+MCAO组(4000m,61.6kPa,3d),每组24只,采用改良Bederson法建立家兔MCAO模型,分别于MCAO前、MCAO后1,3和10d观察脑MRI信号强度、脑梗塞灶体积及梗塞灶边缘PbtO2.结果:MRI结果显示MCAO后3,10d时HBOP+MCAO组和HHP+MCAO组缺血区异常信号范围较单纯MCAO组明显缩小,HBOP+MCAO组缺血区标准化信号强度(SIR)于3,10d时分别为1.62±0.09,1.17±0.10;HHP+MCAO组缺血区SIR于3,10d时分别为1.64±0.06,1.20±0.09;均低于单纯MCAO组的1.83±0.07,1.36±0.06(P<0.05).TTC染色显示的梗塞灶体积在3和10d时HBOP+MCAO组和HHP+MCAO组小于单纯MCAO组;HBOP+MCAO组家兔梗塞灶边缘PbtO2于1,3和10d时分别为(8.27±0.58),(11.92±1.27)和(16.12±1.42)mmHg;HHP+MCAO组家兔梗塞灶边缘Pb-tO2分别为(8.46±0.90),(10.77±1.19)和(15.33±1.29)mmHg,均高于单纯MCAO组的(7.03±1.06),(8.43±0.86)和(11.83±1.28)mmHg(P<0.05).结论:HBOP及HHP对家兔MCAO后局灶性脑缺血具有一定的防治作用.AIM： To observe the extent of infarct and the variations of partial pressure of brain tissue oxygen （ PbtO2 ） around the infracted zone after permanent middle cerebral artery occlusion （MCAO） in rabbits with hyperbaric oxygen preconditioning （ HBOP ） and hypobaric hypoxia preconditioning （ HHP ）. METHODS： Seventy-two male rabbits were randomly divided into 3 groups： simple MCAO group （ n = 24 ）, HBOP ＋ MCAO group （100% 02, 2.5 ATA, 1 h/d, 5 d, n=24） and HHP＋ MCAO group （4000 m, 61.6 kPa, 3 d, n=24）. The signal intensity of MRI, infarct size and PbtO2 were detected at 1, 3 and 10 d after permanent MCAO. RESULTS： Compared with the simple MCAO group, the abnormal signal zone of Ta WI in MRI which indicated ischemia and the TIC staining which indicated infarcted size reduced significantly at 3 and 10 d after permanent MCAO. The Signal intensity ratio （SIR） in the simple MCAO group were （1.83±0.07）, （1.36±0.06） at 3 and 10 d after permanent MCAO, which were significantly decreased after HBOP [（1.62±0.09）, （1.17＋0.10）] and HHP [（1.64±0.06）, （1.20 ± 0.09 ） ] （ P 〈 0.05 ）. PbtO2 around the infraeted regions in the simple MCAO group were （7.03 ± 1.06）, （8.43 ±0.86）, and （ 11.83 ± 1.28） mmHg at 1, 3 and 10d after permanent MCAO, which were significantly increased after HBOP and HHP （P 〈 0. 05 ）. After permanent MCAO, the PbtO2 were （8.27±0.58）, （11.92±1.27）, and （16.12±1.42） mmHg in HBOP group, and （8.46 ±0.90）, （10.77±1. 19）, and （ 15.33±1.29） mmHg in HHP group. CONCLUSION： HBOP and HHP have an preventional and therapic effect on focal cerebral ischemia after permanent MCAO in rabbits.

关 键 词：高压氧 缺氧 局灶性脑缺血 

分 类 号：R743.3[医药卫生—神经病学与精神病学]