Synthesis and Characterization of Chitosan-based Biomateriais Modified with Different Active Groups and Their Relationship with Cytotoxicity  

作　　者：吕颖慧 陈西广 

机构地区：[1]College of Marine Life Sciences,Ocean University of China [2]Institute of Molecular Medicine,Huaqiao University,Quanzhou 362021,China [3]College of Marine Life Sciences,Ocean university of China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2007年第4期695-700,共6页武汉理工大学学报（材料科学英文版）

基　　金：the National Natural Science Foundation of China(No.30370344)

摘　　要：The cytotoxicity profile of three chitosan derivatives with different affinity to water was evaluated in vitro. The derivatives selected were carboxymethylated-chitosan （CMCH）, linoleic acid modifiedchitosan （LACH） and deoxycholic acid modified-chitosan （DACH）, respectively, and the results of FTIR and NMR confirmed the successful modification. Cytotoxicity of these polymers was investigated via the red blood cell lysis assay and the MTT assay. The red blood cell lysis test showed that CH elicited a certain level of red blood cell toxicity, while CMCH, LACH and DACH all displayed low membrane damaging effects, with the hemolysis rates of 2.385%, 1.560% and 4.404%, respectively, which comes well within permissible limit （5%）. The MTT assay revealed that CH exhibited significant inhibitory effect on fibroblast proliferation at higher concentration, while its three derivatives showed no cytotoxicity. CMCH had stimulatory effects on fetal mouse fibroblast proliferation. Differences in cytotoxicity of CH and its derivatives may result from the specific chemical modifications leading to the alteration of molecular charge density and type of the cationic functionalities, structure and sequence, and conformational flexibility.The cytotoxicity profile of three chitosan derivatives with different affinity to water was evaluated in vitro. The derivatives selected were carboxymethylated-chitosan （CMCH）, linoleic acid modifiedchitosan （LACH） and deoxycholic acid modified-chitosan （DACH）, respectively, and the results of FTIR and NMR confirmed the successful modification. Cytotoxicity of these polymers was investigated via the red blood cell lysis assay and the MTT assay. The red blood cell lysis test showed that CH elicited a certain level of red blood cell toxicity, while CMCH, LACH and DACH all displayed low membrane damaging effects, with the hemolysis rates of 2.385%, 1.560% and 4.404%, respectively, which comes well within permissible limit （5%）. The MTT assay revealed that CH exhibited significant inhibitory effect on fibroblast proliferation at higher concentration, while its three derivatives showed no cytotoxicity. CMCH had stimulatory effects on fetal mouse fibroblast proliferation. Differences in cytotoxicity of CH and its derivatives may result from the specific chemical modifications leading to the alteration of molecular charge density and type of the cationic functionalities, structure and sequence, and conformational flexibility.

关 键 词：carboxymethylated-chitosan （CMCH） linoleic acid modified-chitosan （LACH） deoxycholic acid modified-chitosan （DACH） CYTOTOXICITY 

分 类 号：Q50[生物学—生物化学]