草分支杆菌F.U.36混悬注射液对人脐血树突状细胞体外扩增的影响  

Effect of Mycobacterium Phlei F.U.36 Suspended Liquor on Culture and Proliferation of Dendritic Cells Derived from Human Umbilical Cord Blood In Vitro

作　　者：王桂云[1] 孙立荣[1] 

机构地区：[1]青岛大学医学院附属医院儿科,青岛266003

出　　处：《中国实验血液学杂志》2007年第6期1257-1260,共4页Journal of Experimental Hematology

摘　　要：为了探讨草分支杆菌F.U.36混悬注射液(乌体林斯,U)对人脐血来源树突状细胞(DC)体外扩增有无影响,应用Ficoll-Hypaque法分离人脐血单个核细胞(CB-MNC),对照组以RPMI1640培养液诱导培养CB-MNC,实验组分为Utilin"s"组(仅加入Utilin"s"PRMI1640),GTI组(GM-CSF,TNF-α,IL-4)和GTIU组(GM-CSF,TNF-α,IL-4和Utilin"s")。在光学显微镜下观察DC生长情况。至培养第10天,应用流式细胞仪检测各组细胞免疫表型,并将细胞涂片行瑞氏-姬姆萨染液染色,在油镜下观察摄片。结果显示:3个实验组均得到一定数量的典型DC;Utilin"s"组CD1a+、HLA-DR+细胞比例高于对照组;GTIU组HLA-DR+细胞比例升高最明显,高于GTI组。结论:草分支杆菌F.U.36混悬注射液不仅能促进脐血DC体外扩增,还能协同rhGM-CSF、rhTNF-α、rhIL-4促进DC成熟。To investigate the effect of mycobacterium phlei F. U. 36 suspended liquor （ Utilin“s”, U） on the culture and proliferation of dendritic cells （DCs） derived from human umbifical cord blood in vitro, the mononuclear cells （MNCs） were isolated from human umbilical cord blood and cultured with RPMI 1640 in the control group. Test groups consisted of Utilin“s” group （ only Utilin“s” ）, GTI group （ GM-CSF, TNF-α, IL-4 ） and GTIU group （ GM-CSF, TNF-α,IL-4 and Ufilin“s” ）. MNCs in all test groups were cultured with RPMI-1640. The growth of DCs was observed by the light microscopy, the phenotypes of DCs were determined by flow cytomelxy on the 10th day of culture, and some harvest cells were stained with Wright-Giemsa, then observed and photographed under the oil immersion objective. The results showed that the test groups all displayed some number of typical DCs; both CDIa positive cell rate and HLA-DR positive cell rate of the Ufilin“ s” group were higher than those of the control ; HLA-DR positive cell rate of GTIU group increased most significantly and much higher than that of the GTI group. It is concluded that mycobacterium phlei F. U. 36 not only promotes the proliferation of DCs derived from human umbilical cord blood/n vitro, but also co-oper- ates with rhGM-CSF, rhTNF-α and rhlL-4 in promoting the maturity of DCs.

关 键 词：草分支杆菌 树突状细胞 脐血 体外扩增 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]