MMP-2靶向siRNA对成釉细胞瘤裸鼠移植瘤的抑制作用  

Inhibition against xenografts of ameloblastoma in nude mice using MMP-2 targeting siRNA

作　　者：曾东林[1] 黄洪章[2] 张磊涛[2] 张彬[2] 潘朝斌[2] 

机构地区：[1]中山大学附属口腔医院口腔放射科,广东广州510055 [2]中山大学附属第二医院口腔颌面外科,广东广州510120

出　　处：《中国口腔颌面外科杂志》2007年第6期440-444,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(30471896);广东省自然科学基金(04300340;06021272)~~

摘　　要：目的:构建裸鼠皮下人成釉细胞瘤移植瘤模型,研究MMP-2靶向siRNA对成釉细胞瘤侵袭性的抑制作用。方法:取新鲜成釉细胞瘤组织标本,剪切成1～2mm3组织小块,接种于裸鼠前肢根部的背侧皮下。实验分为3组:空白对照组不加任何处理因素;脂质体组注射脂质体混合物;siRNA组注射MMP-2靶向siRNA表达质粒混合物。组织块接种后第2周末开始施加处理因素,记录移植瘤体积和实验终止时的肿瘤重量。采用SPSS10.0统计软件包对数据进行t检验,并对移植瘤进行组织病理学分析。结果:所有移植瘤均成活,病理证实移植瘤为成釉细胞瘤,siRNA组和对照组之间肿瘤的体积有显著性差异,P<0.05。结论:裸鼠皮下接种成釉细胞瘤组织块构建成釉细胞瘤移植瘤动物模型是可行的,MMP-2靶向siRNA可在体内抑制成釉细胞瘤的侵袭性和生长。PURPOSE： To construct subcutaneous xenografts of human ameloblastoma in nude mice and to study the inhibition against the invasiveness of ameloblastoma using RNA interference. METHODS： Fresh ameloblatoma tissue was minced into 1-2 mm^3 in size and subcutaneously transplanted into the back of the forefoot of nude mice. Control groups were designed as follows blank group, in which nothing was done except transplanted with ameloblastoma tissue lump; lipofectamin group, in which the compounds of lipofectamin and plasmid was injected; experimental group, in which MMP-2 specific siRNA expression plasmid was injected around the xenografts. Two weeks after transplantation of the ameloblastoma tissue lump, the experimental factor was exerted on the control group and experimental group according to the study design. The volume and the weight of xenografts in the termination time of the experiment were recorded. Student＇s t-test in SPSSll.0 software package was performed to analyze of the acquired data. Pathological examination was carried out to study the changes in the xenografts. RESULTS： All xenografts survived during the experimental period. The xenografts were identified as ameloblastoma by pathological, examination. There was significant difference of the volume between the control group and the experimental group, P〈0.05. CONCLUSION： It is feasible to construct xenograft of human ameloblastoma by subcutaneously transplanted ameloblastoma tissue lumps into nude mice. The invasiveness of ameloblastoma was inhibited by MMP-2 specific RNA interference.

关 键 词：基因治疗 siRNA MMP-2基因 移植瘤 

分 类 号：R739.8[医药卫生—肿瘤]