锌转运体ZNT1在人阿尔茨海默病大脑的定位  

The Localization of ZincTransporter1(ZNT1) in Alzheimer's Disease Brain

作　　者：张丽红[1] 王辛[1] 郑玮[1] 于丹[1] 荣明[1] 王占友[1] 

机构地区：[1]中国医科大学基础医学院组织胚胎学教研室

出　　处：《解剖科学进展》2007年第4期310-313,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30670722;30770680);高等学校博士学科点专项科研基金(20060159001)

摘　　要：目的研究锌转运体-1(zinc transporter 1,ZNT1)在阿尔茨海默病(Alzheimer's Disease,AD)尸检大脑皮层内的定位分布,探讨ZNT1影响脑锌平衡从而参与AD发病的可能机制。方法应用免疫组织化学、免疫荧光和共聚焦激光扫描显微镜观察ZNT1蛋白和β-淀粉样蛋白(β-amyloid,Aβ)在AD患者大脑内的分布和二者之间的位置关系。结果ZNT1免疫阳性反应产物主要定位于AD大脑皮层老年斑内。几乎所有的Aβ老年斑内均有不同程度ZNT1表达。ZNT1和β-淀粉样蛋白免疫双标结果进一步证实ZNT1免疫产物与Aβ共存于AD患者的老年斑内。结论AD患者大脑皮质Aβ老年斑内有大量ZNT1蛋白表达,提示ZNT1可能参与AD大脑皮质Aβ老年斑的形成。Objective To investigate the localization of ZNT1 in Alzheimer＇s diseased （AD）brain, and to explore the possible role of ZNT1 in AD pathogenesis. Methods Immunohistochemistry, immunofluorescence and confocal laser scanning microscopy were used to analyze the distributions of ZNT1 and β -amyloid protein（A β ）and the relationship between these two proteins in AD brain. Results ZNT1 immunoreactive product was seen to be localized in the senile plaques within the cerebral cortex of AD patients.Almost all of A β senile plaques presented ZNTI immunoreactivity with different expression levels.Confocal microscopic results revealed that a colocalization of ZNT1 and A β was found in the senile plaques. Conclusion The abundant expression of ZNTI in A β plaques suggests that ZNT1 may be involved in the formation of A β senile plaque in AD brain.

关 键 词：人阿尔茨海默病 锌转运体 Β-淀粉样蛋白 老年斑 

分 类 号：R742.5[医药卫生—神经病学与精神病学]