桑黄胞内多糖对白血病细胞K56的增殖抑制效应研究  

Inhibitory Effects of Phellinus Linteus Intracellular Polysaccharide on the Proliferation of Leukemic Cells K562

作　　者：史新强[1] 沈业寿[1] 卫自[1] 马金宝[1] 

机构地区：[1]安徽大学生命科学学院,生化微生物研究所安徽省中药研究与开发重点实验室,安徽合肥230039

出　　处：《癌变．畸变．突变》2007年第6期440-443,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:观察桑黄胞内多糖(PLIP)对体外培养的人白血病细胞K562的增殖抑制作用,并初步探讨其作用机制。材料和方法:采用MTT法及台盼蓝拒染法测定细胞增殖抑制率和生长曲线,Hoechst_PI双荧光染色,流式细胞术和DNA琼脂糖凝胶电泳检测细胞凋亡。结果:PLIP在25μg/ml、50μg/ml、100μg/ml、200μg/ml、400μg/ml、800μg/ml剂量时均能显著抑制K562细胞增殖(P<0.05),其中400μg/ml剂量时抑制率最高,达52.55%,半数抑制浓度(IC50)为262.36μg/ml,流式细胞仪检测PLIP在100μg/ml、200μg/ml、400μg/ml剂量时K562细胞凋亡率分别为5.72%、13.57%、19.39%,并能诱导K562细胞出现凋亡所具有的形态学及生化特征。结论:PLIP对体外培养的人白血病细胞K562生长增殖具有明显的抑制作用,其作用机制可能与诱导K562细胞凋亡和影响细胞周期有关。BACKGROUND ＆ AIM： To examine the inhibitory effects of Phellinus linteus intracellular polysaccharide （PLIP） on proliferation of leukemic cell line K562, and to explore its preliminary mechanisms. MATERIALS AND METHODS： The suppression of cellular growth was detected by MTY assay and trypan blue rejection. Apoptosis was studied by using Hoechst-PI fluorescence staining, DNA agarose gel electrophoresis, and flow cytometry. RESULTS： The PLIP could significantly inhibit the proliferation of K562 cells （P 〈 0.05）, and the highest inhibition rate could reach 52.55% at the dose of 400 μg/ml,IC50 was 262.36 μg/ml. The apoptosis rates for 100 μg/ml,200 μg/ml,400 μg/ml were 5.72%, 13.57%, 19.39%, respectively, and morphologic and physiologic changes of apoptosis in K562 cells were induced . CONCLUSION： PLIP could significantly inhibit proliferation of K562 cells, and the mechanism responsible for the effect might involve the induction of apoptosis and the effects on cell cycle.

关 键 词：桑黄 胞内多糖 K562 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]