咖啡因代谢物AFMU,1X的测定及其在N-乙酰化分型中的应用  

Determination of caffeine metabolites of AFMU, 1X and their application in N acetyltransferase phenotyping

作　　者：曹晓梅[1] 卢建丰[1] 蒋永宏 卓海通[1] 凌树森[1] 

机构地区：[1]南京军区南京总医院临床药理科

出　　处：《中国药学杂志》1997年第8期486-489,共4页Chinese Pharmaceutical Journal

基　　金：国家自然基金

摘　　要：目的：建立一套测定咖啡因的２种主要代谢物ＡＦＭＵ和１Ｘ的高效液相色谱（ＨＰＬＣ）方法。方法：选用岛津ＳｈｉｍＰａｃｋＣＬＣＯＤＳ柱，流动相为甲醇乙腈水醋酸（１２∶１．５∶８６．４５∶０．０５）。对１２０名健康志愿者服用咖啡５ｇ后４ｈ～５ｈ尿样进行测定，以ＡＦＭＵ与１Ｘ摩尔比作为代谢指标，并作频谱显示。结果：受试者可明显划分为快乙酰化者和慢乙酰化者，快、慢乙酰化表型之比１０１∶１９。结论：咖啡因ＨＰＬＣ方法的建立及在Ｎ乙酰化分型中的成功应用。OBJECTIVE: To establish a high performance liquid chromatographic (HPLC) method for determining two major metabolites of caffeine, 5 acetylamino 6 formylamino 3 methyluracil (AFMU) and 1 methylxanthine (1X). METHOD: A Shim Pack CLC ODS column was used, and the mobile phase consisted of methyl alcohol acetonirile water acetic acid (12∶1.5∶86.45∶0.05). Each of 120 volunteers provided a spot sample of urine between 4 and 5 hours after taking 5 g coffee, and the molar ratio of AFMU to 1X was determined. RESULTS: The molar ratio of AFMU to 1X afforded segregation of two apparent modes of acetylation capacity. Nineteen slow acetylators were found among 120 volunteers. CONCLUSION: The established HPLC method for determining the AFMU, 1X is stable and can be used in segregation of phenotype of N aceyltransferase.

关 键 词：咖啡因 代谢物 高效液相色谱 N-乙酰化表型 

分 类 号：R971.7[医药卫生—药品] R965.2[医药卫生—药学]