骨肉瘤与人白细胞抗原关联的初步研究  

A primary study of the association of human leucocyte antigen and osteosarcoma

作　　者：张伟滨[1,2] 沈才伟[1,2] 蔡体栋[1,2] 杨钰琴[1,2] 姚芳娟[1,2] 范丽安[1,2] 

机构地区：[1]上海第二医科大学附属瑞金医院骨科上海市伤骨科研究所 [2]上海第二医科大学上海市免疫学研究所遗传免疫实验室

出　　处：《中华肿瘤杂志》1997年第4期309-312,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金

摘　　要：目的探索中国汉人骨肉瘤与人白细胞抗原（ｈｕｍａｎｌｅｕｃｏｃｙｔｅａｎｔｉｇｅｎ，ＨＬＡ）相关性。方法应用补体依赖微量淋巴细胞毒技术，检测骨肉瘤组２５例汉族患者及对照组２５０例健康汉人的ＨＬＡ－Ａ、Ｂ、ＤＲ、ＤＱ座位抗原，统计比较患者与对照组各座位抗原频率。结果骨肉瘤组ＨＬＡ－Ｂ３５抗原频率为０．４００，对照组为０．０４８，携此抗原的中国汉人骨肉瘤发病的相对危险率是无此抗原者的１３．２２０倍（Ｐ＜０．０１）。含ＨＬＡ－Ｂ１３抗原的患者预后不良的相对危险率为无此抗原患者的１２．０４８倍（Ｐ＜０．０５）。既携ＨＬＡ－Ｂ１３又含ＨＬＡ－Ｂ３５抗原患者，更表现出预后差的趋势。而含ＨＬＡ－Ｂ４０抗原患者的预后较无此抗原患者，相对安全率为７．０５７倍（Ｐ＜０．０５）。结论ＨＬＡ－Ｂ３５与中国汉人骨肉瘤易感性基因紧密连锁，ＨＬＡ－Ｂ１３和ＨＬＡ－Ｂ４０抗原则可能分别与骨肉瘤恶性基因及抵抗性基因相关联。Objective To study the association of human leucocyte antigen and osteosarcoma in Chinese Han nationality. Methods The frequencies of HLA-A,B,DR,DQ locus antigens were tested in a group of 25 osteosarcoma patients and in a control group of 250 healthy persons by using complement dependent microlymphocytotoxity technique. The members of both groups were of Chinese Han nationality. Their results were compared statistically. Results The frequency of HLA B35 was 0.400 in patient group and 0.048 in the control group. The relative risk of suffering from osteosarcoma in persons carrying HLA B35 was 13.220 times as high as that in those without this antigen ( P <0 01). Patients with HLA-B13 had 12.048 fold increase in relative risk with poor prognosis compared to those without this antigen ( P <0 05). A tendency of worst prognosis was seen in patients who carried both HLA B13 and HLA B35, but patients with HLA B40 had 7.057 times better relative safety for good prognosis than those without that antigen ( P <0 05). Conclusion HLA B35 is in close linkage to osteosarcoma susceptible gene in Chinese Han nationality. HLA B13 and HLA B40 may be seperately related to the malignant and resistant genes of ostosarcoma.

关 键 词：骨肉瘤 白细胞抗原 骨肿瘤 

分 类 号：R738.103[医药卫生—肿瘤]