HPLC测定菊苣药材中的有效成分  

Contents determination of effective components in Cichorium intybus by HPLC

作　　者：罗嫄[1] 刘晟[1] 方鲁延[2] 王建芬[1] 李芳[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学分析测试中心,四川成都610041

出　　处：《华西药学杂志》2007年第6期671-672,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的测定菊苣药材中有效成分的含量。方法采用HPLC法。结果秦皮甲素、秦皮乙素、山莴苣苦素、羽扇豆醇的回收率分别为102.01%、102.99%、102.86%、101.06%,RSD分别为2.84%、2.60%、1.12%、2.47%,其回归方程的相关系数分别为0.9995、0.9998、0.9996、0.9992。结论所用方法准确、快速、简便、重复性好,可用于菊苣有效成分的含量测定。OBJECTIVE To determine the contents of target components in Cichorium intybus. METHODS HPLC was adopted. RESULTS The recovery rates of aesculin, aesculetin, lupeol and lactucopicin were 102. 01%, 102. 99% , 102. 86% , 101.06% with the RSD of 2. 84%, 2. 60% , 1.12% and 2.47% , respectively. Correlation coefficients of the 4 components＇ regression equation were 0. 9995, 0. 9998, 0. 9996 and 0. 9992, respectively. CONCLUSION The method is accurate, simple, reproducible, and can be utilized to determine the contents of C. intybus.

关 键 词：菊苣 秦皮甲素 秦皮乙素 山莴苣苦素 羽扇豆醇 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]