3个黄牛品种的myostatin 5′调控区多态与生长性状的相关分析  

Association Analysis on Myostatin 5′Regulatory Region Polymorphism and Growth Traits in Three Cattle Breeds

作　　者：张润锋[1] 陈宏[1] 雷初朝[1] 张春雷[1] 张海军[2] 鲍斌[2] 陈付英[1] 王轶敏 牛晖 王新庄 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室 [2]徐州师范大学细胞与分子生物学研究所,徐州221116 [3]河南省肉牛工程技术研究中心,郑州450003

出　　处：《畜牧兽医学报》2007年第12期1273-1278,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划项目(2006AA10Z197);国家科技支撑计划(2006BAD01A10-5);国家自然科学基金(30771544);河南省杰出人才创新基金项目(0521001900);西北农林科技大学拔尖人才支持计划项目;徐州师范大学专项基金资助

摘　　要：用PCR-RFLPs方法对南阳牛、秦川牛、郏县红牛3个品种共411个个体的myostatin5′调控区的单核苷酸多态性(SNPs)进行分析。结果表明,3个品种在myostatin5′调控区T→A突变以等位基因T为主,仅在郏县红牛中发现1个AA型纯合体,郏县红牛品种显著偏离Hardy-Weinberg平衡状态(P<0.05),其它品种处于Hardy-Weinberg平衡状态(P>0.05)。对黄牛myostatin5′调控区T→A突变与南阳牛、秦川牛和郏县红牛生长性状进行相关分析,结果显示,TT型南阳牛6月龄的胸围和胸围指数显著低于TA型个体,TT型南阳牛18月龄的体高显著高于TA型个体(P<0.05),但基因型对秦川牛和郏县红牛生长性状的效应不显著(P>0.05)。提示在南阳牛的育种实践中应该综合考虑南阳牛生长阶段和所要选择的性状。Single nucleotide polymorphisms （SNPs） of myostatin 5′regulatory region in Nanyang, Qinchuan and Jiaxian Red cattle breeds were analyzed by PCR-RFLPs. The results showed that the allele T was dominant in the three breeds for the T→A mutation, and only one homozygous AA individual was detected in Jiaxian Red cattle which was at Hardy-Weinberg disequilibrium （P〈0.05）. The Nanyang and Qinchuan populations were at Hardy-Weinberg equilibrium （P〉 0.05）. The effects of the T→A mutation on growth traits of the three breeds were also estimated. The results showed that six-month Nanyang cattle with genotype TT had lower heart girth and heart girth index than individuals with TA genotype, eighteen-month Nanyang cattle with TT genotype had higher withers heights than animals with TA genotype （P〈0.05）, but the effects of myostatin genotypes on growth traits were not significant in Qinchuan cattle and Jiaxian Red cattle. These results indicated that the growth stages and the selected traits should be comprehensively considered in the breeding practice of Nanyang cattle.

关 键 词：MYOSTATIN 5′调控区 突变 生长性状 黄牛 

分 类 号：S823.81[农业科学—畜牧学]