用原位杂交技术对牛组织中FMDV RNA检测和定位  

Detection and Localization of the Viral RNA in Bovine Tissues Experimentally Infected with Foot-and-mouth Disease Virus with in situ Hybridization

作　　者：邵军军[1] 常惠芸[1] 林彤[1] 丛国正[1] 独军政[1] 谢庆阁[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,兰州730046

出　　处：《畜牧兽医学报》2007年第12期1341-1344,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重大基础研究"973"项目(2005CB523201)资助;国家支撑计划项目(2006BAD06A03)

摘　　要：采用原位杂交技术对6头试验牛易感染组织中的FMDVRNA进行了检测和定位。结果显示，接毒后7～28d4头牛舌上皮组织、7～21d的喉咽部有强阳性染色；接毒后35d的牛及正常对照牛舌上皮没有阳性染色；接毒牛及对照牛的扁桃体、淋巴结和蹄叉等其他组织中均没有阳性染色，表明牛舌上皮和喉咽部上皮组织中感染了FMDV，本研究为进一步阐明FMDV在宿主体内的持续感染机理提供极有价值的线索。RNAs of Foot-and-mouth disease virus in bovine tissues from six experimentally infected cattle was localized and detected by in situ hybridization. Results showed that strong positive staining was viewed in four bovine tongue epithelial tissues 7-28 days post-infection. The strong positive staining particles were observed in the epithelial tissues of larynx and pharynx 7-21 days post-infection. The positive staining was not observed in the tongue epithelial tissues from 35 days post-infection cattle and the normal control cattle. No positive staining was viewed in the tissues from tonsils, lymph nodes and cuneus ungulae of all six experimental cattles. Results indicated that epithelium of tongue, larynx and pharynx were infected by foot-and-mouth disease virus. The results are very valuable and helpful to further elucidate persistent mechanisms of footand-mouth disease virus in cattle.

关 键 词：FMDV 原位杂交 牛 

分 类 号：S858.235.3[农业科学—临床兽医学]