猪链球菌2型多重PCR检测方法的建立及应用  

Establishment and preliminary application of multiplex PCR for rapid detection of Streptococcus suis serotype 2

作　　者：覃芳芸[1] 熊毅 盛宇明[2] 白昀[2] 覃伦[2] 朱伟[2] 李华明 潘杰[2] 龙剑明[2] 陈磊[2] 刘棋 

机构地区：[1]广西兽医防疫检疫站,广西南宁530001 [2]广西大学动科院,广西南宁530005

出　　处：《畜牧与兽医》2007年第9期4-6,共3页Animal Husbandry & Veterinary Medicine

基　　金：广西科技厅科技攻关与新产品试制项目(0632002-1)

摘　　要：设计3对引物,分别扩增链球菌属特异性gdh、猪链球菌种特异性16S rRNA和猪链球菌2型特异性cps2J等基因,目的片段大小分别为725bp、523bp和387bp。利用合成的3对引物并通过对反应条件与反应体系的优化建立了多重PCR。应用该多重PCR检测了分离到的链球菌1105株,检出猪链球菌667株,猪链球菌2型33株。研究结果表明,该方法特异性高、敏感性强,可广泛应用于猪链球菌病的快速诊断及流行病学调查。Three pairs of primers were designed to amplify three genes, Streptococci genus specific gene （gdh）, Streptococci suis species specific gene （ 16S rDNA） and Streptococci suis type 2 gene （cps2J）. The PCR products were 725 bp, 523 bp and 387 bp, respectively. A multiplex PCR was established by optimizing the reaction conditions and used to detect 1 179 samples. The result showed that there were 667 strains of S. suis and 33 strains of S. suis type 2 in 1 105 strains of Streptococcus detected. A sensitive and specific multiplex PCR assay was established for the application in diagnosis and epidemiological survey of S. suis infection.

关 键 词：猪链球菌2型 三重PCR 检测 

分 类 号：S858.28[农业科学—临床兽医学]