静息细胞法转化天麻素的研究  

A study on microbial transformation of gastrodin by resting cell of Absidia coerulea

作　　者：朱宏莉[1] 张嘉[1] 宋纪蓉[2] 陈邦[1] 孙伟[2] 马震宇[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西北大学化工学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2007年第1期73-76,共4页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(20571060);陕西省科技攻关基金资助项目(2005K16-G73);陕西省教育厅专项基金资助项目(06JK189);西北大学科研基金资助项目(04NW38)

摘　　要：目的研究微生物细胞对天麻素的转化作用。方法采用静息细胞转化法,对天麻素进行转化,并通过理化性质和波谱分析鉴定转化产物的结构。结果转化产物经NMR,EI-MS等的测定证明为天麻苷元(对羟基苯甲醇)。结论利用兰色梨头霉Absidia coerulea3.338 9的静息细胞转化天麻素,在底物质量浓度为2.8 mg/mL,pH 6,28℃条件下转化48 h,转化率可达95.7%。Aim To study the transformation mechanisms of gastrodin by microorganism. Methods Gastrodin was biocatalyzed into p-hydroxy-benzyl alcohol using resting cell techniques. The product was obtained by chromatography, and characterized on the basis of its physical and spectral data. Results The product was identified as p-hy- droxy-benzyl alcohol on the basis of its 1 H, 13 CNMR and El-MS spectral data. Conclusion Gastrodin was biotransformed by resting cell of Absidia coerulea. The optimal transforming condition was investigated： 20 g resting cell （wet weight） incubated at 28℃ for 48 hours, in a 500 mL flask containing 150 mL of medium, resulted in a product with a transformation rate closed to 100 per cent by adding 2. 8 mg/mL substrate.

关 键 词：微生物转化 静息细胞 天麻素 对羟基苯甲醇 

分 类 号：Q81[生物学—生物工程]