孜然芹下胚轴胚性愈伤组织的诱导及再生植株的研究  

Research on Embryonic Callus Induction and Regenerative Plant of Hypocotyl of Cuminum cyminum L.

作　　者：高杰[1] 陈小华[1] 高翔 孙军[1] 

机构地区：[1]新疆农业大学园艺学院,乌鲁木齐830052 [2]新疆维吾尔自治区种子总站,乌鲁木齐830038

出　　处：《新疆农业科学》2007年第6期866-869,共4页Xinjiang Agricultural Sciences

基　　金：国家教育部"春晖计划"项目(Z2005-1-65004)

摘　　要：以孜然芹下胚轴为外值体,研究了在不同种类和浓度激素处理下产生愈伤组织,经继代增殖成胚性愈伤组织,在分化培养基上,胚性愈伤组织进一步分化,得到了再生植株。结果表明:下胚轴在光照时间16 h/d,MS+2,4-D 0.5 mg/L的愈伤组织诱导启动培养基中,20 d愈伤诱导率达100%,继代培养基为MS+NAA 0.5mg/L+6-BA 2 mg/L培养基中,增殖率达80%,在MS+6-BA 3.0 mg/L+NAA 0.5 mg/L培养基中光照16 h/d培养30 d,可见再生植株,再生率为18.75%。The callus were induced from hypocotyls of Cuminum cyminum L.. The rate of the callus induction of hypocotyl culture was 100% for a period of 20d with 16 hour photoperiod each day on MS basal medium supplemented with 2,4 - D 0.5 mg/L. In the second culture, the rate of growth of callus could reach 80% with NAA 0. 5 mg/L and BA 2.0 mg/L. The plant generation was founded on MS basal medium supplemented with 6 - BA 3.0 mg/L ＋ NAA0.5 mg/L, the rate was 18.75%.

关 键 词：孜然芹 下胚轴 愈伤组织诱导 再生植株 

分 类 号：S573[农业科学—作物学]