脂肪分化相关蛋白基因多态性与优质鸡屠宰性状和肌内脂肪含量的相关性研究  

Association between single nucleotide polymorphism of ADFP gene and carcass traits and intramuscular fat content in chicken

作　　者：赵小玲[1] 刘益平[1] 李亮[1] 蒋小松[2] 杜华锐[2] 朱庆[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014 [2]四川省畜科院

出　　处：《遗传》2007年第12期1483-1490,共8页Hereditas（Beijing)

基　　金：四川省"十一五"畜禽育种攻关项目资助(编号:2006YZGG-19)~~

摘　　要：对脂肪分化相关蛋白(Adipocyte Differentiation-Related Protein,ADFP)基因的外显子进行SNPs检测,探讨其作为鸡脂肪性状候选基因的可能性。实验以四川省畜牧科学研究院和大恒家禽育种有限公司培育的优质肉鸡新品系为素材,采用PCR-SSCP的方法进行SNPs检测和基因型的分析。结果找到3个SNPs位点:4 079位由A→T(位点A)、4 843位由C→T(位点B)和7 070位由A→G(位点C)。单位点基因型对屠宰性状的遗传效应分析表明,位点A的基因型对腿肌率、腹脂重、腹脂率和肌内脂肪含量有显著性影响(P<0.05),位点B的基因型对活重和屠体重均有显著性影响(P<0.05),位点C的基因型对胸肌重和肌内脂肪含量有显著性影响(P<0.05),对胸肌率有极显著性影响(P<0.01)。初步推断ADFP基因可能是影响鸡脂肪性状的主效基因或与主效基因连锁,推测可以利用多态位点A和C对鸡腹脂重、腹脂率和肌内脂肪含量进行标记辅助选择。In order to investigate the effect of ADFP gene on the carcass traits and intramuscular fat content of Da-heng high-quality chicken, we analyzed the coding sequence of the ADFP gene by single-strand conformation polymorphism （SSCP） and DNA sequencing in five pure lines and three crossbreds. We found three single nucleotide polymorphism sites, including A/T at base position 4 079 （locus A）, C/T at base position 4 843 （locus B） and A/G at base position 7 070 （locus C）. GLM analysis for the effect of genotypes on carcass traits and intramuscular fat content demonstrated a significant effect on leg muscle percentage, abdomen fat weight, abdominal fat percentage and intra- muscular fat content in locus A （P 〈 0.05）, a significant association with living body weight and carcass weight for locus B （P 〈 0.05） and a significant association with breast muscle weight and intramuscular fat content （P 〈 0.05）, and＇a very significant association with breast muscle percentage for locus C （P 〈 0.01）. It was concluded that ADFP gene may be a major gene or linked to such a gene that affects the fat traits of birds. Polvmorohic loci A and C couldbe used as molecular markers for abdominal fat weight, abdominal fat percentage and intramuscular fat content in birds in future studies.

关 键 词：鸡 脂肪分化相关蛋白基因 屠宰性状 肌内脂肪含量 SNPS 

分 类 号：S831[农业科学—畜牧学]