长程惊厥发作后不同龄期大鼠神经元坏死与血清NSE相关性研究  

A study of correlation between neuron death and NSE after prolonged seizure in immature and mature rats

作　　者：胡湛棋[1] 蒋莉[1] 陈琼[1] 张晓萍[1] 

机构地区：[1]重庆医科大学附属儿童医院,重庆400014

出　　处：《儿科药学杂志》2007年第6期10-12,共3页Journal of Pediatric Pharmacy

基　　金：国家自然科学基金资助项目(30271382)

摘　　要：目的：探讨长程惊厥发作后血清神经元特异性烯醇化酶（NSE）与神经元死亡的相关性．比较其在不同年龄期大鼠神经元死亡的差异。方法：分别在30只成年鼠和30只幼龄鼠中采用美解眠全身注射，建立长时程惊厥大发作模型。分别于惊厥发作停止后4、12、24、48、72h和7d共6个时间点处死5只动物，采用HE染色观察海马及皮层神经元病变。ELISA法测定血清NSE浓度变化。结果：经15—20min惊厥发作后：（1）成年组和幼年组大鼠均出现选择性海马神经元死亡，两组均于惊厥后24—72h达高峰，但成年组死亡神经元数峰值[（190±29）个]是对照组[（38±8）个]的5倍，幼龄组[（98±48）个]与对照组[（33±7）个]比较差异无统计学意义；（2）两组大鼠血清NSE浓度均增高，于惊厥后48h达高峰，成年组血清NSE峰值（5．13±0．77）nmol/L明显高于惊厥前（3．58±0．87）nmol／L，幼龄组（4．41±0．93）nmol／L与惊厥前（3．90±0．09）mol／L比较差异无统计学意义。结论：血清NSE浓度与神经元坏死间存在正相关性。无论脑组织病理学检查与血清NSE浓度监刹均提示长程惊厥发作能产生海马神经元损伤。与成年鼠相比．未成年组对惊厥后脑损伤的耐受性较强。Objective： To explore the relationship of serum neuron specific enolase （NSE） with neuron injury after prolonged seizures and the difference on NSE level between damaged brains of the immature rats and the mature ones. Methods： Prolonged seizures were evoked by Megimide in 30 immature rats and 30 mature ones. Every five rats were killed at the time of 4,12,24,48,72 hours and 7 days after seizure stopped. The dead neurons were observed with HE staining in the areas of hippocampus and eortexes. At the same time points, the NSE level was tested with ELISA. Results： （1） There was a obvious selective neuron death after 15 - 20 min seizures in both groups of the immature and the mature and the peaks were reached in 24 - 72 hours after seizures in both groups. The number of dead neurons after seizures in the brain of the mature （ 190 - 29 cells） was 5 times higher than that of the control group （38 ± 8 cells）, but there was no statistical significance between that of the immature group （98 ± 48 ceils） and the control group （33 ± 7 cells）. （2） By ELISA study, NSE levels of both immature and mature groups increased after seizures and reached peak concentration at 48 hours. The NSE peak concentration after seizures in the mature group （5.13 ± 0.77 nmol/L） was much higher than that before seizures （3.58 ± 0.87 nmol/L）, but no statistical significance was found in the immature group （4.41 ± 0.93 nmol/L） after and before seizures （3.90 ± 0.09 nmol/L）. Conclusions： The results show that prolonged seizures can induce neuron death in hippeeampus and the change of NSE level in sera has a correlation with the number of dead neuron. And compared to mature brains, the immature brains have less damage after prolonged seizures.

关 键 词：长程惊厥 脑损伤 神经元特异性烯醇化酶 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]