南瓜疏松愈伤组织诱导的研究  

Studies on Pultaceous Callus Induction in Pumpkin

作　　者：邹克琴[1] 张拥军[1] 楼纪东[1] 丰金平[1] 曹恬[1] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018

出　　处：《北方园艺》2007年第12期201-203,共3页Northern Horticulture

基　　金：浙江省科技厅计划资助项目(2005C24009)

摘　　要：获得生长迅速,疏松的愈伤组织是进行植物细胞悬浮培养的前提。研究以"粟冠"南瓜子叶做外植体,在添加不同浓度的BA和NAA的MS固体培养基上诱导南瓜愈伤组织,比较南瓜愈伤组织的诱导率和长势,其中当外植体在MS+1.0mg/L6-BA+0.1mg/LNAA的培养基上培养时,愈伤组织诱导率和长势最好。Rapidly vegetal and pultaceous callus is the basis of plant cell suspension culture. Using the leaf blade of pumpkin as explants,added different kinds of hormones and proportions to MS medium for inducing the callus of pumpkin, then compared the induction rate, properties and growth potential. The results showed that the callus induction rate and growth potential were the highest when MS＋1. 0 mg/L BA＋0. 1 mg/L NAA was used.

关 键 词：南瓜 愈伤组织 诱导率 

分 类 号：S642.603.69[农业科学—蔬菜学]