缺氧缺血性脑损伤新生鼠脑组织内一氧化氮合酶阳性终末分布的变化  

Study on distribution of nitric oxide synthase containing terminals in neonatal rat cerebral cortex with hypoxic-ischemic encephalopathy

作　　者：吕剑平[1] 吕进泉[1] 吴丽华[1] 

机构地区：[1]江苏大学附属医院儿科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2007年第6期475-477,共3页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究新生鼠在缺氧缺血性脑损伤(hypoxic-ischemic encephalopathy,HIE)时一氧化氮合酶(nitric ox-ide synthase,NOS)阳性终末在大脑皮质分布数量的变化以及它们与皮质微血管的关系。方法:以10只7日龄SD新生鼠建立缺氧缺血性脑损伤模型(HIE组),于模型制作后即刻取鼠脑固定,然后将大脑皮质作冠状冷冻切片,NAD-PH-d组化染色,计数大脑皮质内NOS阳性终末及附着于血管壁的NOS阳性终末数目;对照组为10只同日龄SD新生鼠。结果:与对照组相比,HIE组大脑皮质NOS阳性终末数量显著增加(P<0.01),且分布至血管壁的终末亦增加(P<0.01)。结论:大脑皮质内的NOS阳性终末纤维参与了缺氧缺血性脑损伤时皮质微循环的调节。Objective： To study the changes of distribution of nitric oxide synthase （NOS） containing terminals in neonatal rat cerebral microvessels with hypoxic-ischemic encephalopathy （HIE）. Methods： The models of HIE of 7-day newborn SD rats were established, then they were perfusion-fixed, their brains were sectioned and stained by NADPH-d histochemistry. The numbers of NOS containing terminals in cere- bral cortex and NOS containing terminals associated with the cerebral microvessels were measured, which were compared with that of normal control group. Results： The result showed that, compared with normal rats, the numbers of NOS containing terminals were markedly increased in cerebral cortex with HIE （P 〈 0.01 ）. Furthermore, the numbers of NOS containing terminals associated with the cerebral microvessels were also increased（P 〈0.01 ）. Conclusion： The NOS containing terminals play partly a role in the regu- lation of the cerebral microcirculation in brain injury caused by hypoxia.

关 键 词：一氧化氮合酶 神经终末 微血管 缺氧缺血性脑损伤 

分 类 号：R743.31[医药卫生—神经病学与精神病学]