鱼肉结合态MCLR的亚慢性毒性研究  

Subchronic Toxic Effects of Fish Muscle-Bound MCLR

作　　者：杨帆[1] 肖邦定[2] 陈晓国[1] 吴幸强[2] 刘旻[2] 

机构地区：[1]武汉理工大学资源与环境工程学院,武汉430070 [2]中国科学院水生生物研究所,武汉430072

出　　处：《环境科学》2007年第12期2811-2815,共5页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2002CB412300);国家高技术研究发展计划(863)项目(2005AA60101005)

摘　　要：为了揭示含有微囊藻毒素水产品的食用安全性,以雄性BALB/C小鼠为模式生物,通过为期13周的灌胃染毒实验,研究了鱼肉结合态MCLR对小鼠的亚慢性毒性作用,并与水溶态MCLR的毒性进行了对比.结果表明,68.75μg/kg(以单位体重计)剂量水溶态MCLR可引起小鼠肝脏显著系数增加(p<0.05),ALT、AST活性增强(p<0.01),引起肝细胞出现有空泡状病变;而肾脏酶学指标BUN、Cr及肾脏组织病理学观察均未发现明显异常;与之相比,同剂量鱼肉结合态MCLR对小鼠各项指标的影响并不明显,仅在实验第1周出现小鼠肝脏肿大(p<0.05)、ALT活性增强(p<0.01).由此推断,鱼肉结合态MCLR的毒性低于水溶态MCLR.Subchronic oral gavage toxicity of MCLR in water and in fish muscle was examined in male Balb/C mice for 13 weeks to assess the safety of aquatic products. The results showed that the liver coefficient （p 〈 0.05 ）, the activities of ALT and AST （p〈0.01） increased significantly and distinct centrilobular to midzonal hepatucellular occurred after oral garage of dissolved MCLR at a dose of 68.75 μg/kg （body weight）, but neither influence on the activities of BUN and Cr nor histological changes on kidney were observed at any time point. In contrast, the administration of fish muscle-bound MCLR at the same dose resulted in no obvious subchronic toxicity in mice, except that the increase of liver coefficient（p〈 0.05）and the activity of ALT（ p 〈 0.01 ）can be observed only at the first week. It was concluded that the toxicity of fish muscle-bound MCLR was much lower than that of dissolved MCLR.

关 键 词：微囊藻毒素 水产品 亚慢性 毒性 

分 类 号：X171.5[环境科学与工程—环境科学]