辛硫磷降解菌XSP-1的分离、鉴定及其降解特性研究  

Isolation,Identification and Characteristics of a Phoxim-Degrading Bacterium XSP-1

作　　者：沈雨佳[1] 洪源范[1] 洪青[1] 蒋新[2] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095 [2]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008

出　　处：《环境科学》2007年第12期2833-2837,共5页Environmental Science

基　　金：国家自然科学基金项目(30400013);江苏省科技厅项目(BG2005322);江苏省教育厅项目(JHZD06-2);土壤与农业可持续发展国家重点实验室开放基金项目(055112);中国博士后基金项目(2005037747);江苏省博士后科研计划项目

摘　　要：从农药厂污泥中分离到1株能以辛硫磷为唯一碳源生长的细菌,命名为XSP-1.根据其生理生化特征和16S rRNA基因序列相似性分析,将该菌株初步鉴定为戴尔福特菌属(Delftiasp.).该菌株能在7 h内完全降解100 mg/L的辛硫磷.XSP-1降解辛硫磷的最适pH为7.0,最适温度为35℃,降解速率与初始接种量呈正相关,该菌株对甲基对硫磷、毒死蜱、杀螟硫磷也有较好的降解能力.根据已报道有机磷农药降解基因mpd的保守序列设计引物,未从该菌株中扩增到目标条带,但是该菌株是否带有新的有机磷农药降解基因仍需要进一步研究.A bacteria strain XSP-1 capable of utilizing phoxim as sole carbon source was isolated from sludge collected from a pesticide manufacturer. It was preliminarily identified as Delfiia sp. according to its physiological-biochemical analysis and the similarity analysis of its 16S rRNA gene sequence （GenBank Accession No. EF061135）. Strain XSP-1 could degrade 100 mg/L phoxim within 7 h completely. The optimal pH and temperature for degradation were 7.0 and 35℃ respectively. The degrading rate showed a positive correlation to the initial inoculum size. Strain XSP-1 also showed good degrading performance against methyl parathion, chlorpyrifos and fenitrothion. PCR detection with degenerated primers designed according to the conserved sequences of reported mpd gene did not find target band, but it needs further study to verify whether strain XSP-1 harbors a new mpd gene.

关 键 词：辛硫磷 生物降解 戴尔福特菌属 XSP-1 

分 类 号：X172[环境科学与工程—环境科学]