小麦胚乳醇溶蛋白反相高效液相色谱(RP-HPLC)分离方法的建立  

Suitable RP-HPLC for wheat gliadin in endosperm cells

作　　者：赵龙刚[1] 宋希云[1] 史雪辉[1] 张恩盈[1] 王兆华[1] 

机构地区：[1]莱阳农学院生命科学学院,山东青岛266109

出　　处：《现代仪器》2007年第6期78-80,共3页Modern Instruments

基　　金：学校博士创新基金(630408)

摘　　要：通过对比试验研究洗脱时间、柱温、流速、洗脱梯度等对反相高效液相色谱法的影响,确立适宜小麦胚乳醇溶蛋白(Gliadin)反相高效液相色谱法(RP-HPLC)分离的最佳实验方法为:在1.0mL/min的流速、60℃的柱温条件下对上样醇溶蛋白进行洗脱、洗脱梯度为流动相B的体积比在55min内由21%升至48%(V/V),最后通过210nm紫外光检测洗脱组分的吸收值。Studies about the effect of evolution time, column temperature, flow, evolution grades, extraction for protein on reversed-phase high performance liquid chromatorgraphy against wheat gliadin in endosperm cells by contrasting experiment had been done, and the suitable RP-HPLC for wheat gliadin in endosperm cells was set up. he results showed that the optimal analytical parameters were ： eluting gliadin compenents under the condition of flowing speed 1.0 mL/min, column temperature 60℃ and the variable concentrations of evlution B growing from 21% to 48% （v/v） in 55 minutes,at last testing absorbance at 210nm.

关 键 词：小麦 醇溶蛋白 反相高效液相色谱法 

分 类 号：O657.72[理学—分析化学]