乙醇对兔角膜上皮细胞活性影响的实验研究  

Experimental Study on the Vitality of Corneal Epithelieum after Exposed to 20% Alcohol for Different Time

作　　者：马义宾[1] 刘艳娣[2] 

机构地区：[1]泰山医学院附属泰安医院眼科医院,山东泰安271000 [2]莱阳农学院校医院,山东莱阳265200

出　　处：《泰山医学院学报》2007年第1期12-13,共2页Journal of Taishan Medical College

摘　　要：目的研究20%乙醇不同浸润时间,对兔角膜上皮细胞活性的影响,为LASEK术中选择适宜的乙醇浸润时间提供理论依据。方法10只实验兔按每组2只随机分为5组:正常对照组、20S组、30S组、40S组、50S组,实验组分别行20%乙醇浸润20 s、30 s、40 s、50 s。角膜标本分别行酶组织化学染色观察。结果与正常对照组相比,20%乙醇浸润兔角膜上皮细胞20 s、30 s,细胞活性无明显降低(P>0.05);浸润时间一旦达到或超过40 s,细胞活性明显降低(P<0.001)。结论LASEK术中20%乙醇的最适浸润时间以不超过30 s为宜。Objective To evaluate the vitality of corneal epithelieum after exposed to 20% alcoholic solution for different time. Methods： According to the time of rabbits sacrifice, 10 healthy adult New Zealand white rabbits were devided into 5 groups randomly. They were fresh control group, 20S group,30S group,40S group and 50S group . In experimental groups the eyes were exposed to 20% alcoholic solution for 20s ,30s ,40s or 50s. Enzyme histochemistry methods with VIDAS image analysis system were used to evaluate the metabolic enzyme activities ,lactate dehydrogenase （LDH） and succinate dehydrogenase （SDH）. Resuhs：Contrast with the fresh control group , the corneal epithelial cells which were infiltrated by 20% alcohol for 20s or 30s, remained satisfactory vitality, （ P 〉 0.05 ） ; While those were infiltrated for 40sor 50s, had been weakened deeply, （ P 〈 0.001 ）. Conclusion ： The density of alcoholic solution and the time to be exposed for were very important to laser epithelial keratomileusis（LASEK）. 20% alcoholic solution last not more than 30 seconds will be more successful.

关 键 词：准分子激光上皮瓣下角膜磨镶术 乙醇 代谢 活性 

分 类 号：R779.63[医药卫生—眼科]