Bcl-2和Bax蛋白在糖尿病大鼠耳蜗中的表达  

Expressions of Bcl-2 and Bax protein in cochlea of diabetic rats

作　　者：杜波[1] 王苹[1] 王宇声[1] 杜宝东[1] 

机构地区：[1]吉林大学第一医院耳鼻咽喉-头颈外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第6期968-970,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：世界卫生组织(WHO)资助课题(H-16-133PBD)

摘　　要：目的:探讨糖尿病耳聋大鼠的耳蜗病变与凋亡相关蛋白Bcl-2和Bax表达的相关性。方法:35只大鼠随机分为糖尿病组和对照组。糖尿病组(25只)采用STZ腹腔一次性注射制备,对照组(10只)注射等量生理盐水。在成模后3个月时采用免疫组织化学方法,对大鼠耳蜗组织进行Bcl-2和Bax的表达检测。结果:糖尿病大鼠耳蜗结构未见异常的形态学改变。对照组大鼠耳蜗内、外毛细胞及血管纹、螺旋神经元均有少量的Bcl-2表达,糖尿病组仅血管纹组织表达的Bcl-2明显高于正常组,毛细胞和螺旋神经元的表达与正常对照组比较差异无显著性(P>0.05)。计算机定量分析显示,糖尿病组和对照组耳蜗血管纹的Bcl-2表达灰度值分别为135.32±21.69和45.32±9.08,两组比较差异有显著性(P<0.01)。糖尿病组与对照组大鼠耳蜗组织均不表达Bax蛋白。结论:糖尿病大鼠血管纹组织表达过量的Bcl-2蛋白可能是糖尿病耳蜗早期病变的影响因素之一。Objective To investigate the correlation of cochlear injury and expressions of Bcl-2 and Bax in the cochlea of diabetic rats. Methods Thirty five Wistar rats were randomly divided into diabetic group （n=25） and control group （n= 10）. Diabetic deafness was induced in rats by intraperitoneal injection of streptozotocin, while the rats in normal group were injected with normal saline. Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in the cochlea after 3 months. Results No significantly morphological change in cochlea of diabetic rats was found. The Bcl-2 levels in stria vascularis in diabetic group and control group were 135.32±21.69 and 45.32±9.08,respectively, there was significant difference （P〈0.01）. There were no changes of Bax protein expression in hair cells, spiral ganglion and stria vascularis between diabetic group and control group. Conclusion Over-expression of Bcl 2 protein in stria vaseularis may be involved in the early stage of diabetic cochlear damage.

关 键 词：BCL-2蛋白 BAX蛋白 糖尿病 实验性 耳蜗 

分 类 号：R-332[医药卫生] R587.1