抑胶素对大鼠C6胶质瘤细胞血管内皮生长因子的抑制作用  

Inhibitory effect of antigliomatin on expression of VEGF in rat C6 brain glioma cells

作　　者：崔俐[1] 林卫红[1] 杨弋[1] 徐丹[2] 王绍[3] 张淑琴[1] 

机构地区：[1]吉林大学第一医院神经内科,吉林长春130021 [2]长春大学医院内科,吉林长春130022 [3]吉林大学基础医学院生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第6期989-991,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20050407-6)

摘　　要：目的:探讨抑胶素对大鼠C6胶质瘤细胞血管内皮生长因子(VEGF)的抑制作用。方法:采用单层贴壁培养法对大鼠C6脑胶质瘤细胞株进行培养,并用2、4、8和16 ng.L-1抑胶素作用72 h,免疫组化法观察抑胶素对C6胶质瘤细胞VEGF的抑制作用。结果:VEGF表达阳性的细胞绝大部分表现为细胞浆和(或)细胞核染成黄褐色。PBS对照组的C6胶质瘤细胞VEGF在细胞浆中表达明显,经不同浓度的抑胶素处理72 h后,肿瘤内VEGF阳性细胞密度较PBS对照组下降(P<0.05,P<0.01),不同浓度的抑胶素作用后C6细胞中VEGF灰度值不同:2 ng.L-1抑胶素组(182.3±5.7)与PBS对照组(185.6±6.5)比较差异有显著性(P<0.05),4、8和16 ng.L-1抑胶素组及顺铂处理组细胞VEGF灰度值(分别为176.8±5.4、169.8±4.1、162.6±2.9和167.3±3.5)与PBS对照组比较差异有显著性(P<0.01)。结论:抑胶素对大鼠C6胶质瘤细胞VEGF有抑制作用。Objective To study the inhibitory effect of antigliomatin on the expression of vascular endothelial growth factor （VEGF） in rat C6 brain glioma ceils. Methods The rat brain glioma ceils was cultivated with the stationary culture technique. The different rat C6 brain glioma ceils groups were treated with 2, 4, 8, and 16 ng ·L^-1 antigliomatin for 72 h in vitro and then the inhibitory effect of antiglioma on the expression of VEGF was observed with immunohistochemical method. Results The cytoplasm and （or） nucleus of most VEGF positive ceils were stained to yellow brown. VEGF expressed significantly in cytoplasm of the rat C6 glioma ceils in control group. The density of the VEGF positive ceils was decreased in glioma compared with control group after treated with antigliomadn for 72 h （P〈0.05, P〈0.01）, and the antigliomatin with differenct concentrations resulted in the different densities of VEGF, there was no difference between the group treated with 2 ng ·L^- 1 antigliomatin （182.3±5.7） and control group （185.6±6.5） （P〈0.05）. The densities of the VEGF in the groups treated with 4, 8, 16 ng ·L^-1antigliomatin and CDDP （176.8±5.4, 169.8±4.1, 162.6±2.9, 167.3±3.5） were significantly lower than that in control group （P〈0.01）. Oonclusion Antialiomatin has inhibitory effect on the expression of VEGF in rat C6 glioma cells.

关 键 词：抑胶素 神经胶质瘤 内皮生长因子 

分 类 号：R-332[医药卫生] R730.264