间充质干细胞移植脑缺血区GDNF及受体表达变化  

Expressions of GDNF,GFRα1 and c-Ret in rat middle cerebral artery occlusion model following mesenchymal stem cells transplantation

作　　者：李昕华[1] 邵延坤[1] 郭丽[2] 钱佳利[3] 莽靖[1] 徐忠信[1] 

机构地区：[1]吉林大学中日联谊医院神经内科,吉林长春130033 [2]吉林大学公共卫生学院卫生毒理学教研室,吉林长春130021 [3]长春中医药大学基础医学院生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第6期1043-1046,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20030536-5)

摘　　要：目的:观察骨髓间充质干细胞(MSCs)移植对大鼠大脑中动脉栓塞(MCAO)后胶质细胞源性神经营养因子(GDNF)及其受体GFRα1、c-Ret表达的影响。方法:用线栓法建立大鼠MCAO模型,随机分为MSCs移植组、盐水组和假手术组。移植后1、3、5和7 d应用免疫组织化学和原位杂交技术检测GDNF及其受体的表达。结果:与盐水组和假手术组比较,移植组缺血周边区可见GDNF阳性表达细胞增多。移植后第7天移植组GDNF在缺血周边区的表达高于盐水组和假手术组(P<0.05)。移植组的皮层、海马及底节等部位均可见GFRα1、c-Ret mRNA的阳性表达细胞,且多见于细胞形态完整的神经元,范围集中在缺血周边区。随时间延长,GFRα1、c-Ret mRNA表达细胞增多,在第7天时表达最多,与其他两组比较差异有显著性(P<0.05)。结论:MSCs移植入脑组织可能分泌和(或)促进神经细胞分泌GDNF,并增加缺血周边神经元表达GDNF及其受体,从而起到脑保护作用。Objective To observe the expressions of glial cell line derived neurotrophic factor （GDNF） and it＇s receptor after transplanting mesenchymal stem cells （MSCs） to rats with middle cerebral artery occlusion （MCAO）. Methods Adult Wistar rats were randomly divided into MSCs transplantation group （transplantation group）, equivalence saline injection group （saline group） and sham-operation group. At the 1st, 3rd, 5th, 7th day after transplantation, the expressions of GDNF protein, GFRα1 and c-Ret mRNA were detected by using immunohistochemical and in situ hybridization. Results The GDNF positive cells at the periphery were more than those in ischemic center, and at the 7th day after transplantation of MSCs, the expression of GDNF was higher than those in sham-operation and saline groups （P〈0.05）. The GFRα1 and c-Ret mRNA can be detected in the integrated neurons. At the 7th day, the expressions of GFRα1 and c-Ret mRNA in transplantation group were higher than those in sham-operation and saline groups （P〈0.05） . Conclusion MSCs can secrete and/or promote the nerve cells secrete GDNF, and increase the expression of GDNF receptors in neuron. MSCs may play a great role in the treatment of the central nerve system diseases.

关 键 词：中胚层 干细胞 脑缺血 脑源性神经营养因子 受体 

分 类 号：R743.3[医药卫生—神经病学与精神病学]