MMP-9及其抑制因子TIMP-1在膀胱癌中的表达及意义  

Expressions of matrix metalloproteinases-9 and its tissue inhibitor TIMP-1 in carcinoma of bladder and significances

作　　者：周洪澜[1] 李路江[1] 王医术[2] 王心蕊[2] 廖翔宇[2] 高婷[2] 

机构地区：[1]吉林大学第一医院泌尿外二科,吉林长春130021 [2]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第6期1057-1060,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：教育部留学回国人员科研启动基金资助课题(20041169);吉林省科技发展计划项目资助课题(200505107)

摘　　要：目的:探讨基质金属蛋白酶9(MMP-9)及其抑制因子(TIMP-1)在膀胱移行细胞癌(TCCB)中的表达及意义。方法:应用免疫组织化学和RT-PCR方法检测47例TCCB组织和5例正常膀胱组织MMP-9和TIMP-1蛋白的表达情况。结果:在正常膀胱组织中MMP-9、TIMP-1不表达或呈分布均匀的微量表达,而在TCCB中则有不同程度的阳性表达,二者表达量比较差异均有显著性(P<0.05)。随着TCCB临床病理分期、分级的升高,MMP-9的表达呈现增高趋势(P<0.01),但TIMP-1表达情况与肿瘤临床病理分期、分级无明显关联(P>0.05)。MMP-9和TIMP-1在初发TCCB和复发TCCB中的表达差异均无显著性(P>0.05)。结论:MMP-9在TCCB的发生、发展中具有促进作用;MMP-9和TIMP-1之间的平衡失调可能是肿瘤侵袭和转移的分子机制之一。Objective To study the expressions and significances of matrix metalloproteinases-9 （MMP-9） and tissue-inhibitor of matrix metalloproteinase-1 （TIMP-1） in transitional cell carcinoma of bladder （TCCB）. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of MMP-9 and TIMP-1 in 47 surgical respected specimens from patients with TCCB （experimental group） and 5 normal bladder tissues （control group）. Results MMP-9 and TIMP-1 had different degree expressions in experimental group, but the expressions were weak or negative in control group. Furthermore, the positive expression levels of MMP-9 were significantly increased with stages and grades （P〈0.01）; however the expression of TIMP-1 did not show significantly correlation with different grades and stages of TCCB tissue （P〉0. 05）. Both MMP-9 and TIMP-1 had no significant correlation with firstoccurrent TCCB and recrudescent TCCB. Conclusion MMP-9 plays advance roles in the occurrence and development of TCCB; the unbalance between MMP-9 and TIMP-1 is one of important molecular mechanisms in the aggressive and metastasis biologic behavior of tumor.

关 键 词：膀胱肿瘤/病理学 明胶酶B 金属蛋白酶1组织抑制剂 

分 类 号：R737.14[医药卫生—肿瘤]