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作 者:全世明[1] 高志强[1] 亓放[1] 葛平江[1] 刘稳[1] 冯国栋[1] 查洋[1] 金晓峰[1]
机构地区:[1]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉科,北京100730
出 处:《中国耳鼻咽喉头颈外科》2007年第11期623-626,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery
基 金:国家"十五"科技攻关项目(2004BA720A18-01);科技部科研院所社会公益研究专项基金资助项目(2002DB40097);教育部博士点专项基金资助项目(20060023017)联合资助
摘 要:目的建立免疫缺陷小鼠周围性面瘫模型并结合荧光金逆行示踪技术进行评价,并与相应野生型小鼠模型比较。建立稳定的免疫缺陷小鼠面神经损伤模型评价系统,为进一步揭示外伤性面瘫的神经免疫机制提供模型基础。方法外科无菌手术方法切断裸小鼠、野生型BABL/c小鼠面神经出茎乳孔主干,分别于脑干取材前2天行面神经断端涂抹荧光金示踪剂。面瘫第14天灌注固定动物后,标本自头侧至尾侧行连续冰冻切片,荧光显微镜下观察面神经运动神经元情况。用ImageProPlus5.1软件计数神经元数量。结果面神经轴切损伤后14天,荧光切片面神经运动神经元核团显示清晰,便于计数;裸小鼠组与野生型组神经元数目有显著性差异(分别为2423±20,2748±60,P=0.0011)。结论荧光金逆行示踪技术结合裸鼠面神经损伤模型评价系统是有效、易行的,为进一步揭示外伤性面瘫中以T细胞行为研究为中心的神经免疫机制提供模型基础。OBJECTIVE To establish stable and reliable assessment system on immune deficiency mouse model with facial nerve paralysis by the application and assessment of fluorogold retrotracer on the nude mouse with facial nerve transection and comparing with wild type mouse model, so as to provide a proper animal model for revealing the latent neuroimmunologic mechanism of traumatic facial paralysis. METHODS The nude mouse( BABL/c background) group and the wild type mouse( BABL/c background) group were subjected to a right facial nerve axotomy. Fluorogold retrotracer was used at specific time. After collecting the slices of brain stem two weeks after the operation, the facial motoneuron was observed under fluorescence microscope, then analyzed and counted with the software Image Pro Plus5.1. RESULTS After transection of the facial nerve, the facial motoneuron retrotraced with fluorogold was luminous and easy for counting; There was significant difference of survival facial motoneuron between the nude mouse group and the wild type mouse group (2423±20 vs 2748±60, P=0.0011). CONCLUSION The assessment system of nude mice facial nerve paralysis model together with fluorogold retrotracing method is effective and feasible, which provide a proper animal model for revealing the latent neuroimmunologic mechanism centering on T cell behavior of the pathogenesis and progression of facial nerve trauma.
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