纤溶酶原激活剂抑制物-1基因4G/5G多态性与特发性卵巢早衰的相关性  

Relationship between plasminogen activator inhibitor-1 gene 4G/5G polymorphism and idiopathic premature ovarian failure

作　　者：管立学[1] 高丽[2] 孙静华[3] 王敬先[1] 李海波[1] 王瑞丽[1] 

机构地区：[1]山东省潍坊市人民医院中心实验室,261041 [2]山东省潍坊市人民医院肿瘤内科,261041 [3]山东省潍坊市人民医院遗传科,261041

出　　处：《实用医学杂志》2007年第23期3673-3675,共3页The Journal of Practical Medicine

基　　金：潍坊科技发展计划项目(编号:2004-8)

摘　　要：目的:探讨纤溶酶原激活剂抑制物-1(plasminogen activator inhibitor-1,PAI-1)基因启动子区4G/5G多态性与特发性卵巢早衰(premature ovarian failure,POF)发病的关系。方法:应用PCR-RFLP分析,检测65例特发性POF患者(POF组)和75例正常妇女(对照组)PAI-1基因4G/5G多态性。结果:特发性POF患者组PAI-1基因型频率分布,4G/4G型为29.2%,4G/5G型为55.4%,5G/5G型为15.4%;POF患者组4G/4G基因型频率明显高于对照组(16.0%),但差异无显著性意义(x2=3.54,P>0.05);而4G等位基因频率(0.569)显著高于对照组(0.447)(x2=4.18,P<0.05),携带4G等位基因个体发生特发性POF的相对风险OR=1.64,95%CI1.02～2.64。结论:PAI-1基因4G/5G多态性与特发性POF的发病有关,4G等位基因可能是特发性POF发病的危险因素之一。Objective To investigate the relationship between plasminogen activator inhibitor-i （PAI-1）gene promoter region 4G/5G polymorphism and idiopathic premature ovarian failure （POF）. Methods The 4G/5G polymorphism of PAI-1 gene in 65 patients with POF （POF group） and 75 healthy volunteer （control group） were detected by a combination of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results The genotype frequencies of PAI-1 gene in POF group was 29.2% for 4G/4G, 55.4% for 4G/5G and 15.4% for 5G/5G, respectively. There were no significant differences of the 4G/4G genotype between POF group and control group （X^2= 3.54, P 〉 0.05）,but the 4G allele frequency（0.569） was significantly higher in POF group than that（0.447） in control group（x^2=4.18, P 〈 0.05）. The 4G allele was associated with POF （OR=1.64, 95%CI 1.02-2.64）. Conclusion PAI-1 gene 4G/5G polymorphism may be related with POF, and 4G allele may be one of the major risk factors in the pathogenesis of POF.

关 键 词：卵巢功能早衰 纤溶酶原激活物抑制物1 基因 多态现象 遗传 

分 类 号：R711.75[医药卫生—妇产科学]