人参中人参皂苷的直接高压微波辅助降解  

Degradation of Ginsenosides in Root of Panax ginseng C.A.Mey. by High-pressure Microwave-assisted Extraction

作　　者：王玉堂[1] 李绪文[1] 金海燕[1] 于永[1] 游景艳[1] 张昆[1] 丁兰[1] 张寒琦[1] 

机构地区：[1]吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2007年第12期2264-2269,共6页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20050560)资助

摘　　要：采用高效液相色谱-电喷雾质谱联用法测定了人参提取液中的人参皂苷.考察了天然人参皂苷发生降解的条件,同时研究了单体人参皂苷Rg1,Re,Rb1,Rc,Rb2和Rd的降解,并对降解产物进行了分析.结果表明,随着提取压力的升高,提取液中天然人参皂苷的含量逐渐减少,同时产生多种次级人参皂苷.当微波提取压力达到600 kPa,提取时间为10 min时,提取液中的主要天然人参皂苷达到完全降解,次级人参皂苷Rg3含量达到最高.在单体人参皂苷Rb1,Rc,Rb2和Rd的降解产物中均得到人参皂苷Rg3.During the process of high-pressure microwave extraction, the crude ginseosides in root of Panax ginseng C. A. Mey. （P. ginseng） can be degradated. Some glycosyls can partly be put off and the sub-ginseosides can be obtained. So the sub-ginseosides can be directly extracted from the root of P. ginseng. HPLC-ESI-MS was applied to determine the ginsenosides. The degradation conditions of crude ginseosides were examined and the degradation products of ginseoside Rgl, Re, Rbl, Rc, Rb2 and Rd were also studied. The results indicate that with the increase of the extraction pressure, the contents of the ginsenosides decrease and meanwhile the contents of some low polar sub-ginseosides increase. At the extraction pressure of 600 kPa and the extraction time of 10 min, the crude ginseosides in root of P. ginseng can be completely degradated. The content of sub-ginseosides Rg3 is the highest. The ginsenosides Rg3 can be found in the degradation products of ginseoside Rbl, Rc, Rb2 and Rd.

关 键 词：人参皂苷 降解 微波提取 HPLC-ESI-MS 

分 类 号：O657[理学—分析化学] O629[理学—化学]