异黄酮同分异构体的ESI-IT-TOF质谱特征及区别  

Identification and Mass Spectrometric Characterization of Isomeric Isoflavone Aglycones by ESI-IT-TOF Mass Spectrometry

作　　者：李慧[1] 万乐人 王弘[1] 端裕树 陈世忠[1] 

机构地区：[1]北京大学药学院,北京100083 [2]岛津国际贸易有限公司北京分析中心,北京100020

出　　处：《高等学校化学学报》2007年第12期2284-2289,共6页Chemical Journal of Chinese Universities

基　　金：国家'九七三'计划(批准号:2006CB504707)资助

摘　　要：采用电喷雾-离子阱-飞行时间串联质谱(ESI-IT-TOF)技术,在正/负离子检测模式下对芒柄花素及其同分异构体7甲-氧基异黄酮的质谱裂解规律进行了系统研究.实验结果表明,该化合物在正、负离子模式下均得到了5级高分辨质谱.结果显示,二者在负离子模式下的碎片相同,而在正离子模式下的碎片裂解不同.根据正负离子模式的5级高分辨质谱推导了两者的可能裂解规律,丰富了异黄酮的ESI-MSn数据,为其它异黄酮类化合物的分析鉴定提供了有效的质谱方法.mass spectrometric method based on the combined with electrospray ionization/ion trap/time-of- flight（ESI-IT-TOF） mass spectrometer was used for systematic research of 7-methoxy-4＇-hydroxy isoflavone and formononetin. MS5 spectra both in positive and negative ion modes with a high resolution were obtained. Different product ions were observed in positive ion mode, while almost the same product ions were observed in negative ion mode. With the high selectivity of parent ions and precise formulae of each fragment, fragmentation mechanisms were proposed with a high reliability. The established method could be used for the sensitive and rapid identification of isoflavonoids.

关 键 词：7甲-氧基-4′-羟基异黄酮 芒柄花素 同分异构体 异黄酮质谱特征 离子阱-飞行时间质谱联用仪 

分 类 号：O657.63[理学—分析化学]