Effect and mechanism of β-L-D4A on DNA polymerase α  

作　　者：Yan Li Ju-Sheng Lin Ying-Hui Zhang Xiao-Yan Wang Ying Chang Xing-Xing He 

机构地区：[1]Institute of Liver Diseases, Tongji MedicalCollege, Huazhong University of Science and Technology, 1095Jiefang Street, Wuhan 430030, Hubei Province, China

出　　处：《World Journal of Gastroenterology》2007年第46期6243-6248,共6页世界胃肠病学杂志（英文版）

基　　金：National Natural Science Foundation of China,No.30330680

摘　　要：AIM： To investigate the safety of β-L-D4A on DNA polymerase α.METHODS： Ion exchange chromatography was used to separate DNA polymerase cc from crude extract of human Hela cells. Detailed kinetic parameters were determined for β-L-D4A against DNA polymeraseα . RESULTS： DNA polymerase was purified with 4% yield and 31000 units/mg specific activity. The Michaelis constant （Km = 3.22 i^mol/L）, 50% inhibition concentration （IC50 = 178.49 μmol/L） and inhibition constant （Ki = 126 mol/L） of β-L-D4A were determined by kinetic analysis. CONCLUSION： β-L-D4A is a more safe nucleoside for hepatitis B virus （HBV） infection with a lower host toxicity.AIM:To investigate the safety of β-L-D4A on DNA polymeraseα. METHODS:Ion exchange chromatography was used to separate DNA polymeraseαfrom crude extract of human Hela cells.Detailed kinetic parameters were determined for β-L-D4A against DNA polymeraseα. RESULTS:DNA polymeraseαwas purified with 4% yield and 31000 units/rag specific activity.The Michaelis constant (Km=3.22μmol/L),50% inhibition concentration (IC50=178.49μmol/L) and inhibition constant (Ki=126μmol/L) of β-L-D4A were determined by kinetic analysis. CONCLUSION:β-L-D4A is a more safe nucleoside for hepatitis B virus (HBV) infection with a lower host toxicity.

关 键 词：NUCLEOSIDE Β-L-D4A DNA polymeraseα Kinetic study Side effect Hepatitis B virus 

分 类 号：R512.62[医药卫生—内科学]