铜胁迫对竹叶眼子菜叶片生理指标和超微结构的影响  

Effects of Cu^(2+) stress on leaf physiological indice and ultrastructure of Potamogeton malaianus.

作　　者：计汪栋[1] 施国新[1] 杨海燕[1] 徐勤松[1] 许晔[1] 张慧[1] 

机构地区：[1]南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室,南京210046

出　　处：《应用生态学报》2007年第12期2727-2732,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30370083;30670121);高等学校博士学科点科研基金项目(20050319005);江苏省教育厅自然科学基金资助项目(05KJB180067).

摘　　要：通过溶液培养试验,研究了不同Cu2+浓度(0、2、4、6和8 mg.L-1)胁迫对沉水植物竹叶眼子菜叶片叶绿素含量、可溶性蛋白含量、保护酶活性、活性氧产生以及细胞超微结构的影响.结果表明:随着Cu2+浓度的增加,竹叶眼子菜叶片总叶绿素、类胡萝卜素和可溶性蛋白含量逐渐下降;超氧化物歧化酶、过氧化物酶逐渐下降,而过氧化氢酶则呈先升后降趋势;超氧阴离子产生速率、过氧化氢、丙二醛和可溶性糖含量均呈上升趋势;不连续聚丙烯酰胺凝胶电泳(SDS-PAGE)显示,Cu2+处理后,引起分子量为60.9、18.8、15.7、22.3和30.0 kDa多肽的表达量减少或消失;电镜观察发现,Cu2+对叶片细胞器的超微结构特别是叶绿体、线粒体和细胞核造成严重损伤;Cu2+对竹叶眼子菜的致死浓度范围在2~4 mg.L-1.With solution culture, this paper studied the toxic effects of different concentrations of Cu^2＋ （0, 2, 4, 6, and 8 mg · L^- 1 ） on the chlorophyll content, soluble protein content, protective enzyme activities, active oxygen generation, and uhrastructural changes in Potamogeton malaianus leaf. The results showed that with increasing concentration of Cu^2＋ , the chlorophyll, carotenoid and soluble protein contents and uperoxide dismutase （SOD） and peroxidase （POD） activities decreased, catalase （CAT） activity increased first and decreased then, while O2^τ , H2O2, MDA and soluble sugar content increased. SDS-PAGE revealed that Cu^2＋ induced the disappearance or the decrease of the expression amount of four polypeptides with apparent molecular weights of 60. 9, 18.8, 15.7, 22. 3 and 30. 0 kDa. Transmission electron microscopy observation indicated that the higher Cu^2＋ concentration, the more serious damages the leaf organelles, especially chloroplast, mitochondria and nucleus, suffered. It was concluded that the death of P. malaianus plant under Cu^2＋ stress was resulted from the destruction of physiological function and structural foundation, and the disorder of physiological metabolism. The lethal concentration of Cu^2＋ could be 2-4 mg·L^-1.

关 键 词：竹叶眼子菜 CU^2+胁迫 保护酶 超微结构 

分 类 号：X173[环境科学与工程—环境科学]