用富含DHA的裂殖壶菌对轮虫进行营养强化的研究  

Studies on the DHA enrichment of rotifer with Schizochytrium limanium

作　　者：宋晓金[1] 张学成[1] 朱路英[1] 李荻尔 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]青岛森淼实业有限公司,山东青岛266101

出　　处：《海洋科学》2007年第12期43-46,共4页Marine Sciences

基　　金：青岛市科技发展计划项目(04-2-HH-76)

摘　　要：裂殖壶菌(Schizochytrium limanium)是一种高DHA含量的海洋真菌,本实验用裂殖壶菌对褶皱臂尾轮虫(Brachionus plicatilis)进行营养强化。结果表明:3个实验组强化后轮虫体内的DHA/FA值和DHA含量均显著提高,DHA/FA值分别为5.98%、10.21%和13.44%,轮虫体内的DHA质量比分别为4.42,7.85,8.14 mg/g,并于12 h后达到最大值,超过12h后轮虫体内的DHA质量比及DHA/FA值开始下降。对照组在实验过程中均未检出DHA。各实验组的其他指标,如轮虫密度也显著高于对照组,并且实验组的怀卵率分别为34.8%、46.5%和53.7%,而对照组仅为20.1%。Schizochytrium limanium is a kind of marine fungus with high DHA content. In our studies, rotifer Brachionus plicatilis was enriched with S. limanium, The variation of physiology and ecology of rotifer during the experiments was observed and the DHA in rotifer was evaluated. The results showed that： all of the three enriching groups got better results than the control group. DHA content increased obviously and reached the maximum after 12 h enrichment. The max DHA/FA content of the testing groups reached 5.98% 10.21% and 13.44% respectively,and the max DHA content reached 4.42 mg/g 7.85 mg/g and 8. 14 mg/g respectively. No DHA was detected in the control group in the whole experiments. Other indexes such as rotifer density, egg-hanging rate in the testing groups were also obviously better than those of the control group. Compared with the egg-hanging rates in the control group, 20.1% , the egg hanging rates in the testing groups had reached 34.8%, 46.5% and 53.7% respectively.

关 键 词：营养强化 DHA 褶皱臂尾轮虫(Brachionus plicatilis) 裂殖壶菌(Schizochytrium limanium) 

分 类 号：S963.21[农业科学—水产养殖]