FasL基因修饰骨髓树突状细胞诱导同种异体反应性T淋巴细胞凋亡  

Dentric cells genetically engineered to express Fas ligand induce the apoptosis of allogeneic reactive lymphocvtes

作　　者：张传永[1] 钱晓峰[1] 王科[1] 王平[1] 李相成[1] 张峰[1] 王学浩[1] 

机构地区：[1]南京医科大学第一附属医院肝脏移植中心,210029

出　　处：《江苏医药》2007年第12期1245-1247,共3页Jiangsu Medical Journal

摘　　要：目的探讨FasL基因转染骨髓来源树突状细胞(DC)诱导同种效应T淋巴细胞凋亡及抗原特异性T淋巴细胞的低反应性的机制。方法采用腺病毒载体将FasL基因转染骨髓来源树突状细胞用流式细胞术(FCM)检测FasL基因的表达及同种效应T淋巴细胞的凋亡率,通过混合淋巴细胞反应(MLR)检测抗原特异性T淋巴细胞的低反应性。结果FasL基因通过腺病毒载体转染骨髓来源树突状细胞可以获得有效表达,FasL-DC组的凋亡率为83.19%,明显高于对照组(LacZ-DC组、Day-7-DC组)的11.57%、7.93%(P<0.05),FasL-DC组与对照组相比显著地抑制了淋巴细胞的增埴(P<0.05),但在与无关第三者(C3H)共培养时,T淋巴细胞仍然显示强的增埴能力(P>0.05)。结论FasL基因转染DC能够诱导同种反应性T淋巴细胞凋亡,产生抗原特异性T淋巴细胞的低反应性。Objective To investigate the effect of the dentric cells（DCs） modified by Fas ligand gene on inducing apoptosis and hyporesponsiveness of allogeneic reactive lymphocvtes. Methods DCs were prepared from mouse bone marrow and genetically modified by fas ligand. The expression products and the apoptosis rate of T lymphocytes were detected by flow cytometry （FCM）. The propliferative ability of T lymphocytes with the mixed lympiocyte reaction （MLR）was observed. Results The Fas ligand gene could be transfected into BMDC and expressed efficiently. FCM analysis showed that the apoptosis rate of FasL-DC group（83.19%） of T lympiocytes induced by transfected BMDC was much higher than that of the control （11.57% for LacZ-DC group and 7. 93% for Day-7-DC group,P〈0. 05）. The result of MLR showed that allogeneic T lympiocyte cell proliferation was significantly decreased. But to another alloantigen MLR was not inhibited. Conclusion The DCs transfected with Fas ligand could induce apoptosis of the allogeneic reative T lymphocytes and alloantigen-specific hyporesponsiveness of T lymphocytes cell in vitro.

关 键 词：FAS配体 凋亡 基因转染 树突状细胞 

分 类 号：R394[医药卫生—医学遗传学]