雷公藤多甙对IL-1β诱导大鼠滑膜细胞株RSC-364增殖和IL-6分泌的影响  

Study on the effect of Tripterygium Glycosides on the Proliferation and Secretion of Interleukin-6 of Rat Synovial Cell Line 364 Cultures Induced with IL-1β

作　　者：蔡安季[1] 苏卓娃[1] 马文松[1] 陈群[1] 杨宇[1] 杜冀晖[1] 周蓓[1] 李蓉[1] 麦丽文[1] 易钊泉[1] 

机构地区：[1]广东医学院附属深圳南山医院中心实验室,518052

出　　处：《放射免疫学杂志》2007年第6期546-547,共2页Journal of Radioimmanology

摘　　要：目的:观察雷公藤多甙对IL-1β诱导大鼠滑膜细胞株RSC-364增殖和IL-6分泌的影响,探讨其治疗类风湿性关节炎的作用机理。方法:培养大鼠滑膜细胞株RSC-364,运用CCK-8法检测不同剂量的雷公藤多甙对IL-1β诱导大鼠滑膜细胞株RSC-364增殖的影响,酶联免疫吸附实验检测细胞株培养上清中的IL-6的含量。结果:经过IL-1β诱导大鼠滑膜细胞株RSC-364,在不同浓度的雷公藤多甙的作用48h后,其增殖明显受到抑制,并呈剂量依赖性。同时各剂量组均可抑制IL-1β诱导大鼠滑膜细胞株RSC-364分泌炎性因子IL-6的分泌。结论:雷公藤多甙抑制IL-1β诱导大鼠滑膜细胞株RSC-364的过度增殖和IL-6的分泌,这可能是其治疗类风湿性关节炎的作用机理之一。Objective To explore the treatment mechanism of Tripterygium Glycosides for rheumatoid arthritis with investigating the effect of Tripterygium Glycosides （TG） on the proliferation and secretion of interleukin -6 （IL -6 ） of rat synovial cell line 364 （RSC -364） cultures induced with interleukin - 1 beta （IL - 1 β）. Methods TG （0, 5, 10, 20mg/L） solutions were added into RSC - 364 cultures, the proliferation of RSC - 364 induced with IL - 1 β at different strength of TG was detected with cell counting Kit - 8 （ CCK - 8 ） assay and the expression of IL - 6 in the supernatant was detected with enzyme - linked immunosorbent assays. Resuits The proliferation and the secretion of IL - 6 of RSC - 364 induced with IL - 1 β were significantly inhibited with the addition of different strength of TG and the inhibition was dose - dependent. Gonclusion The proliferation and the secretion of IL - 6 of RSC - 364 induced with IL - 1 β were suppressed by TG, which was possibly related to the mechanism of treatment of RA.

关 键 词：雷公藤多甙 IL-1Β 大鼠滑膜细胞株RSC-364 增殖IL-6 

分 类 号：R742.1[医药卫生—神经病学与精神病学]