聚氨酯泡沫半固定化培养盘基网柄菌  

Immobilized cultivation of Dictyostelium discoideum with polyurethane foam

作　　者：梁兴超[1] 卢英华[2] 陈杰[1] 徐志南[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,杭州310027 [2]厦门大学化学与化学工程系,厦门361005

出　　处：《生物加工过程》2007年第2期52-56,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(30370039)

摘　　要：研究了聚氨酯泡沫应用于固定化盘基网柄菌的可行性,发现以简单处理过的聚氨酯泡沫为载体,能够高效实现盘基网柄菌的固定化培养。考察了载体粒径大小、载体量和摇床转速等对固定化培养的影响,在优化的培养条件和固定化条件下,盘基网柄菌的最大细胞密度是悬浮培养的2-4倍。The immobilization of D. discoideum with polyurethane foam （PUF） was proposed and the effects of PUF＇s properties on the immobilization was examined. The results showed that PUF was a suit- able carrier for the efficient imoblization of D. discoideum. The effects of PUF addition and culture condi- tions were optimized to attain high density of D. discoideum . Under optimized culture conditions, high cell density of D. discoideum （ 4.56 × 10^7 cells/mL） 2 -4 times higher than that of free-cell culture in the has been achieved in the shake flasks, which was axenic medium.

关 键 词：盘基网柄菌 聚氨酯泡沫 固定化培养 

分 类 号：Q939.97[生物学—微生物学]