用反相高效液相色谱法测定南方红豆杉组织培养产物中的紫杉醇  

Determination of Taxol in Tissue Culture of Taxus Mairei by Reversed Phase High Performance Liquid Chromatography

作　　者：赵芳[1] 耿征[1] 刘谦光[1] 万秀琴[1] 陈战国[1] 

机构地区：[1]陕西师范大学化学系,西安710062

出　　处：《分析化学》1997年第8期941-943,共3页Chinese Journal of Analytical Chemistry

基　　金：陕西省科技风险投资公司资助项目

摘　　要：采用反相高效液相色谱法，对南方红豆杉组织培养产物中的紫杉醇含量进行定量分析。在Ｃ１８柱上，以含３％四氢呋喃和７％乙腈的甲醇－水（３：１，Ｖ／Ｖ），为流动相进行分离，检测波长 ２５４ ｎｍ。采用外标法定量，测得紫杉醇在０．０４～０．８ ｍｇ／Ｌ范围内具有良好的线性关系，回收率在９８．２％～１０１．６％之间。该法准确、快速、重现性好，结果令人满意。A new high performance liquid chromatographic method for the determination of taxol in tissue culture of Taxus mairei is described. Taxo1 can be separated on a Sheer C18(5 μm, 5 mm ×150 mm) column at 40°C by using a mobile phase containing 7 % acetonitrile and 3% tetrahydrofuran methanol-water (3: l, V/V) at 0. 6 mL/min flow rate. The samples were quantified with an ultraviolet detector operated at 254 nm. The ca1ibration curve was linear in the range of 0. 04～0. 8 mg/L of taxol with a correlation coefficient of 0. 9996. The relative standard deviation for determination of 0. 4 mg/L taxol was less than l. 8% (n= 6) and the recovery of taxol was 98. 2% ～101. 6 %.

关 键 词：高效液相色谱 红豆杉 组织培养 紫杉醇 TAXOL 

分 类 号：R979.1[医药卫生—药品] R284.1[医药卫生—药学]