双水相体系中甾类化合物6-甲基氢化可的松的生物转化研究  

Study on biotransformation of 6-methlhydrocortisone in aqueous two-phase systems

作　　者：孙新宇[1] 王普[1] 何军邀[1] 周莺[1] 

机构地区：[1]浙江工业大学生物制药研究所,浙江杭州310032

出　　处：《浙江工业大学学报》2007年第6期617-621,共5页Journal of Zhejiang University of Technology

摘　　要：双水相体系作为一种新型生物反应体系,与其他反应体系相比,双水相体系具有生物相容性高、反应条件温和以及实现生物反应-产物分离耦合等优点.利用简单节杆菌Arthrobacter simplex UR016在PEG/Dextran双水相体系中对6-甲基氢化可的松进行Δ^1脱氢制备6-甲基泼尼松龙,对双水相体系的组成进行了选择和优化,研究得到的最佳转化体系组成和配比为16%PEG20000/4%Dextran40000.在构建的此双水相转化体系中最适6-甲基氢化可的松底物加入量为4.5 g/L,转化48 h,6-甲基氢化可的松的转化率可达到95.72%,较水相脱氢转化率提高了25%～30%.As a new type of bioconversion media,aqueous two-phase system has many advantages such as high biological compatibility,mild conditions,substrate and product separation-coupling,compared to other media.The D1 dehydrogenation reaction from 6-methlhydrocortisone to 6-methlprednisolone by Arthrobacter simplex UR016 in aqueous two-phase system,which consists of PEG and Dextran,is investigated.The composition of the aqueous two-phase system is also optimized.The results show that the optimal constituents are 16%PEG20000/4%Dextran 40000,with the bioconversion efficiency of 6-methlhydrocortisone reach to 95.72% at 4.5 g/L substrate concentration and after 48 h conversion.

关 键 词：6-甲基氢化可的松 简单节杆菌 双水相 生物转化 

分 类 号：TQ467.95[化学工程—制药化工]