胸腺肽α_1对脂多糖刺激后人外周血单核细胞和淋巴细胞免疫功能的影响  

Effect of thymosinα_1 on immune function of monocyte and lymphocyte in hman peripheral blood stimulated by lipopolysaccharide

作　　者：杨红明[1] 于燕[1] 雷树红[1] 胡森[1] 柴家科[1] 盛志勇[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100037

出　　处：《感染．炎症．修复》2007年第4期202-204,共3页Infection Inflammation Repair

基　　金：首都医学发展科研基金联合攻关项目(2002-1010)

摘　　要：目的:观察胸腺肽α_1对脂多糖刺激后人外周血单核细胞人类白细胞组织相容性抗原-DR(HLA- DR)表达率及淋巴细胞凋亡率的影响。方法:分离、培养正常人外周血单核细胞和淋巴细胞,空白对照组仅加1640培养液,内毒素(LPS)组加LPS刺激,LPS+胸腺肽α_1组先用不同浓度(100、10、1、0.1、0.01μg/ml)胸腺肽α_1预处理细胞,5min后再加入LPS(100 ng/ml)刺激。培养12 h后用流式细胞术检测CD14^+单核细胞HLA-DR表达率和外周血淋巴细胞凋亡率。结果:LPS单独刺激单核细胞时,CD14^+单核细胞HLA-DR表达率[(11.14±5.09)%]较空白对照组[(74.00±3.50)%]明显降低,用胸腺肽α_1预处理后,HLA-DR表达率随着胸腺肽α_1浓度的增高而增高(P均<0.01)。LPS单独刺激淋巴细胞时,淋巴细胞凋亡率[(8.16±0.51)%]较空白对照组[(4.40±0.53)%]明显增加,而用胸腺肽α_1预处理后,淋巴细胞凋亡率随着胸腺肽α_1浓度的增高而降低,胸腺肽α_1浓度为10μg/ml和100μg/ml时,淋巴细胞凋亡率[(4.65±0.75)%和(4.50±0.36)%]与空白对照组比较差异无显著性(P均<0.01)。结论:胸腺肽α_1对LPS引起的人外周血单核细胞和淋巴细胞免疫功能下调有显著抑制作用。Objective： To observe the effect of thymosin α1 on immune function of monocyte and lymphocyte in human peripheral blood stimulated by lipopolysaccharide（LPS）. Methods： Monocytes and lymphocytes were isolated from peripheral blood of healthy adults. The cells for test were divided into control （cultivated with medium 1640）, LPS stimulation alone （stimulated with 100 ng/ml LPS） and LPS stimulation ＋ thymosin α1 groups. In the last group, different doses （100, 10, 1, 0. 1, 0.01 μg/ml）of thymosin α1 were added followed by LPS（100 ng/ml） challenge. After cultivating for 12 hours, expression of HLA-DR antigen on CD14^＋ monocyte and lymphocyte apoptosis rate were determined by direct immunofluorescence with a flow cytometer. Results： HI.A-DR expression was significantly lower in monocytes with LPS stimulation m（11.14± 5.09）%] than that in cells without LPS stimulation [（74. 00±3. 50）%]. After being pretreated with thymosin α1 , HLA-DR expression was significantly increased in a concentration-dependent manner. Apoptosis rate in lymphocytes stimulated by LPS alone [（8. 16±0.51）%] was significantly higher than those in controls [（4.40±0.53）%]. Apoptosis decreased when pretreated with thymosin α1 , which also showed a concentration-dependent manner. NO significant difference in apoptosis rate of lymphocytes was found between 10 μg/ml or 100 μg/ml LPS stimulation and controls. Conclusion： The results suggest that thymosin μ has a significant effect on LPS induced immunosuppression of human monocyte and lymphocyte.

关 键 词：胸腺肽Α1 内毒素 单核细胞 人类白细胞组织相容性抗原-DR淋巴细胞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]