人类S期激酶相关蛋白2表达质粒的构建  

Construction of expression plasmid of human S-phase kinase-associated protein 2 gene

作　　者：陆建新[1,2] 孙承龙[1] 王熵婵[2] 沈爱国[2] 赵玥铭[2] 刘海鸥[2] 程纯[2] 

机构地区：[1]南通大学附属医院输血科,南通226001 [2]南通大学微生物与免疫学教研室

出　　处：《南通大学学报（医学版）》2007年第4期249-251,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省卫生厅资助项目(H200632);江苏省教育厅资助项目(04KJB320114);江苏省南通市社会发展项目(S2006003)

摘　　要：目的:克隆人类S期激酶相关蛋白2(Skp2)基因,构建其表达质粒,为今后进一步研究Skp2的生物学功能提供基础。方法:采用RT-PCR技术扩增人类Skp2 eDNA全长序列,运用DNA重组技术将其重组于pcDNA3.1/myc- His A质粒,构建Skp2表达质粒。结果:通过酶切电泳分析及DNA测序分析证实,实验成功构建了Skp2表达质粒。结论:Skp2表达质粒pcDNA3.1/mye-His A-Skp2构建成功,为进一步研究Skp2的生物学功能及其在肿瘤发生、发展、诊断和预后中的作用奠定了基础。Objective ：To clone S-phase kinase-associated protein 2（Skp2） gene and construct the expression plasmid to offer ground for further research on the biological function of Skp2. Methods ：The RT-PCR technique was used to amplify the full length of Skp2 cDNA sequences, and the DNA reconstruction technique was used to construct Skp2 expression plasmid by reconstructing it in the plasmid of pcDNA3.1/myc-His A. Result：Successfully constructed plasmid pcDNA3.1/myc-His A-Skp2 was confirmed by the analysis of enzyme-cutting, electrophoretic analysis and DNA sequencing. Conclusion ：Skp2 expression plasmid pcDNA3.1/myc-His A-Skp2 was successfully constructed to establish the ground for further research on the biological function of Skp2 and its role in tumor occurrence, development, diagnosis and prognosis.

关 键 词：S期激酶相关蛋白2 DNA重组技术 细胞周期 

分 类 号：R730.5[医药卫生—肿瘤]