DNase Ⅰ对非黏液型和黏液型铜绿假单胞菌生物膜的影响  

Influence of DNase Ⅰ on Bacterial Biofilm Induced by Nonmucoid or Mucoid Pseudomonas aeruginosa

作　　者：倪明[1] 余冰[2] 田德英[1] 陈红云[3] 李霞[3] 宋佩辉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,武汉430030 [2]华中科技大学同济医学院基础医学院病原生物学系,武汉430030 [3]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《华中科技大学学报（医学版）》2007年第6期776-779,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30571645)

摘　　要：目的研究不同浓度DNase Ⅰ对非黏液型和黏液型铜绿假单胞菌生物膜形成及成熟生物膜的影响。方法分光光度计法检测25、501、00 U/ml的DNase Ⅰ对非黏液型铜绿假单胞菌PAOⅠ和黏液型铜绿假单胞菌PA17生长曲线的影响;改良平板培养法建立生物膜模型,分别在生物膜形成过程中及生物膜形成后用25、50、100 U/ml的DNase Ⅰ处理,扫描电镜观察PAO Ⅰ和PA17的生物膜形态。结果随着DNase Ⅰ浓度增加,PAO Ⅰ和PA17的对数生长期延迟,但最终不能抑制细菌生长;25 U/ml的DNase Ⅰ即可抑制不同类型铜绿假单胞菌生物膜形成且分解成熟生物膜,100U/ml的DNase Ⅰ可完全阻止铜绿假单胞菌生物膜形成并彻底分解成熟生物膜。结论DNase Ⅰ不能直接杀灭铜绿假单胞菌,但可有效抑制铜绿假单胞菌生物膜形成并分解成熟生物膜,对非黏液型和黏液型铜绿假单胞菌生物膜的影响无明显差异,作用效果呈浓度依赖性。Objective To investigate the influence of DNase Ⅰ on the biofilm formation and mature biofilm induced by nonmucoid or mucoid Pseudomonas aeruginosa. Methods Biofilm was established and treated with DNase Ⅰ of different concentrations in the process of biofilm formation and the time point when biofilm was mature. The samples were detected with scanning electron microscope. Results 25 U/ml DNase Ⅰ could inhibit the biofilm formation by nonmucoid or mucoid Pseudomonas aeruginosa and disintegrate mature biofilm. 100 U/ml DNase Ⅰ could prevent the biofilm formation and disintegrate mature biofilm completely. Conclusion DNase Ⅰ can inhibit the biofilm formation by nonmucoid or mucoid Pseudomonas aeruginosa and disintegrate mature biofilm effectively in a concentration-dependent manner.

关 键 词：DNASE Ⅰ 铜绿假单胞菌 生物膜 

分 类 号：R378.991[医药卫生—病原生物学]