γ干扰素对实验性肝纤维化大鼠Smad3 mRNA的影响  

Effects of IFN-γ on Expression of Smad3 mRNA in Liver Tissue of Rats with Hepatic Fibrosis

作　　者：孙校男[1] 娄国强[1] 王先开[1] 

机构地区：[1]杭州市第六人民医院,310014

出　　处：《医学研究杂志》2007年第12期55-57,F0003,共4页Journal of Medical Research

摘　　要：目的初步探讨γ干扰素抗纤维化的分子机制。方法SD大鼠70只,模型对照组和γ干扰素治疗组注射40%CCl4油剂按0.3ml/100g皮下注射,2次/周,另设正常对照组大鼠10只。γ干扰素治疗组造模同时肌内注射γ干扰素0.2MU/kg,1次/日,模型对照组及正常对照组肌内注射生理盐水,连续干预12周。第12周末处死所有大鼠。取肝脏标本行常规苏木素-伊红和胶原染色,在光镜观察肝组织炎症活动度和肝纤维化情况并进行评分;实时荧光定量PCR方法检测大鼠肝脏Smad3mRNA的表达。结果肝组织炎症活动度和肝纤维化评分显示γ干扰素治疗组大鼠炎症程度、肝纤维化程度与模型对照组大鼠的肝组织炎症程度、肝纤维化程度比较显著改善(P<0.01,P<0.01)。荧光定量RT-PCR检测显示:与正常对照组Smad3mR-NA相比,模型对照组大鼠肝脏中Smad3mRNA表达显著增加(P<0.01);与模型对照组相比,γ干扰素组大鼠肝脏组织中Smad3mRNA表达明显减少(P<0.05)。结论γ干扰素抗实验性肝纤维化作用与下调Smad3mRNA来实现。干预转化生长因子β1信号转导过程是γ干扰素抗肝纤维化的机制之一。Objective Study the possible mechanisms of inhibiting hepatic fibrosis of IFN -γ by observing the effects of IFN - γ on the expression of smad3 mRNA in rat hepatic fibrosis model. Methods 70 rats were divided into three groups at random - fibrosis model group,IFN -γ treatment group and normal control group. There are 30 rats in fibrosis model group which were induced to hepatic fibrosis by subcutaneous injection of carbon tetrachloride（ CCI4 ） for 12 weeks. In the meantime, using IFN -γ to treat the rats in IFN -γ treatment group for 12 weeks. Histopathology changes and degree of fibrosis in livers of all rats were observed. Eventually, Smad3 mRNA were detected and quantified by real - time RT - PCR. Results In contrast with normal control group, the degrees of fibrosis in rat fibrosis model were significantly increased （P 〈 0.01 ） and the expression of Smad3 mRNA were also significantly increased in rat fibrosis model（ P 〈0.01 ）. It was found that the degrees of fibrosis and Smad3 mRNA level in IFN -γ treatment group were all significantly lower than those in fibrosis model group （ P 〈 0.01 ,P 〈 0.05, respectively）. Conclusions IFN - γ can decreased the expression of Smad3 mRNA in liver, which may be one of mechanisms of IFN -γ inhibiting hepatic fibrosis.

关 键 词：Γ干扰素 肝纤维化 实验性 荧光定量RT—PCR 转化生长因子Β1 Smatd3蛋白 

分 类 号：R575.2[医药卫生—消化系统]