α干扰素对结肠癌细胞株lovo和sw1116中X染色体连锁凋亡抑制相关因子1表达调节作用  

The role of interferon-α in regulating the X-linked inhibitor of apoptosis protein-associated factor 1 expression in lovo and sw1116 cells

作　　者：徐迪晖[1] 王继德[1] 程天明[1] 姜泊[1] 杨洲[1] 张宇生[1] 

机构地区：[1]南方医科大学南方医院消化病研究所

出　　处：《医学研究生学报》2007年第12期1242-1244,1247,共4页Journal of Medical Postgraduates

基　　金：广东省自然科学基金资助项目(批准号:7005164)

摘　　要：目的:研究α干扰素(IFN-α)对结肠癌细胞株lovo和sw1116中X染色体连锁凋亡抑制相关因子1(XAF1)表达调节作用。方法:以不同浓度(250、125、62.5和0 U/ml)的IFN-α作用细胞,荧光素酶报道系统检测IFN-α对XAF1启动子活性的影响,运用逆转录聚合酶链反应(RT-PCR)、蛋白质免疫印迹等方法观察IFN-α对XAF1的mRNA和蛋白表达水平的影响。结果:IFN-α可提高XAF1启动子活性,在lovo和sw1116中分别达到61.7%和87.1%(P<0.05),同时在mRNA和蛋白水平上调XAF1表达,并呈剂量依赖关系(P<0.05)。结论:IFN-α可上调XAF1表达,提示XAF1可能是干扰素刺激基因(ISG)。Objective：To investigate the role of interferon-α（IFN-α） in regulating the XAF1 expression in lovo and swl 116 cells. Methods：The cells were treated with 0,62.5,125 and 250 unit of IFN-α, XAF1 expression detected by RT-PCR and immunoblotting, and the XAF1 promoter activity determined by luciferase reporter assay. Results：IFN-αupregulated XAF1 expression at mRNA and protein levels, and increased XAF1 promoter activity by 61.7% and 87.1% in lovo and swl 116 cells respectively, both in a dose-dependent manner. Conclusion：IFN-α can increase XAF1 expression, and XAF1 may be an interferon stimulating gene （ISG）.

关 键 词：X染色体连锁凋亡抑制相关因子1 抑癌基因 Α干扰素 干扰素刺激基因 

分 类 号：R735.3[医药卫生—肿瘤]