水貂肠炎细小病毒结构蛋白VP2基因的克隆和序列分析  

Cloning and Sequencing of VP2 Gene of Structural Protein Mink Enteritis Parvovirus

作　　者：闫喜军[1] 张海玲[1] 柴秀丽[1] 吴威[1] 易立[1] 王凤雪[1] 邵西群[1] 罗国良[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2007年第4期1-3,6,共4页Special Wild Economic Animal and Plant Research

基　　金：科技部科研院所社会公益研究专项(2005DIB4J048)

摘　　要：对水貂肠炎细小病毒SMPV-11株结构蛋白VP2基因进行扩增,获得了基因序列全长为1755bp的SMPV-11株VP2基因,其编码584个氨基酸;应用DNAStar分析软件对该序列进行同源性分析发现,SMPV-11株VP2基因与其他6株水貂肠炎细小病毒株核苷酸和氨基酸均有较高的同源性,分别为99.2%～99.4%和98.6%～99.1%,与猫泛白细胞减少症病毒标准株CU-4株同源性为99.3%和99.1%,系统发生树分析表明它们属于同一个分支。本研究为水貂肠炎细小病毒分子流行病学调查和新型疫苗的研究奠定基础。According to the complete genome sequence of mink enteritis parvovirus in GenBank, a pair of primers were designed to amplify the VP2 gene of MEV SMPV-11 strain. It contained an open reading frame of 1755bp that encoded 584 amino acids. Sequence homology was analyzed with DNAStar software, and results indicated SMPV-11 VP2 gene shared higher homology with other six MEV isolations, the homology of nucleotide and deduced amino acids were 99.2 %-99.4% and 98.6 % - 99.1%, respectively, and 99.3% and 99.1% with FPV CU-4 strain,phylogenetic tree showed that these stains were belonged to a branch. This study provided the basis evidence for molecular epidemiology investigation and research of new-type vaccines of MEV.

关 键 词：水貂肠炎细小病毒 VP2基因 序列分析 

分 类 号：S865.22[农业科学—野生动物驯养] S852.61[农业科学—畜牧兽医]