室旁核内血管紧张素Ⅱ对大鼠胃缺血/再灌注后胃黏膜NF-κB表达的影响  

Effect of angiotensinⅡ in the paraventricular nucleus on the expression of NF-κB in gastric mucosa following gastric ischemia/reperfusion in rats

作　　者：胡霞[1] 张咏梅[1] 石玥[1] 张建福[1] 

机构地区：[1]徐州医学院生理学教研室徐州医学院神经生物学研究中心,江苏徐州221002

出　　处：《徐州医学院学报》2007年第12期771-774,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30570671);江苏省教育厅科学研究基金(05KJB310134);徐州医学院科研课题(07KJ58)

摘　　要：目的研究室旁核(paraventricular nucleus,PVN)内注射血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)对大鼠胃缺血/再灌注(gastric ischemia/reperfusion,GI/R)后的胃黏膜核因子κB(NF-κB)表达的影响。方法采用核团微量注射法以及夹闭大鼠腹腔动脉30 min后松开动脉夹血流复灌1 h的GI/R模型,计数胃黏膜损伤指数并应用免疫组化方法检测胃黏膜NF-κB p65的表达。结果①GI/R可引起胃黏膜损伤,PVN内微量注射AngⅡ(0.3μl含30 ng)后GI/R损伤显著减轻;侧脑室注射(icv)AngⅡ受体拮抗剂洛沙坦,能阻断AngⅡ对GI/R损伤的保护作用,使GI/R损伤明显加重;②正常大鼠胃黏膜可见NF-κB表达,并主要在胞质表达;GI/R后,NF-κB表达增多,尤其是胞核表达明显增加;PVN内微量注射AngⅡ后,可使胃黏膜NF-κB表达量减少;icv给予洛沙坦可阻断AngⅡ的效应,即GI/R损伤后胃黏膜NF-κB阳性细胞数表达量增加。结论PVN内微量注射AngⅡ对大鼠GI/R损伤有明显的保护作用,且该作用可被洛沙坦翻转;NF-κB在PVN内微量注射AngⅡ减轻大鼠GI/R损伤过程中可能发挥着重要作用。Objective To study the effect of angiotensin Ⅱ （ Ang Ⅱ ） in the paraventricular nucleus （PVN） on the expression of NF -κB in gast,ic mucosa following gastric ischemia/reperfusion （GI/R） in rats. Methods Intranuclear microinjection and model of GI/R were employed in the experiment. The GI/R model was established by clamping the celiac artery for 30 rain to induce ischemia and allowing reperfusion for 1 h. The animals were finally sacrificed to investigate the gastric mucosal damage index and the expression of NF -κB in the gastric mucosa by immunohistochemistry. Results ① GI/R induced gastric mucosal injury; microinjection of Ang Ⅱ （30 ng）into PVN obviously attenuated the GI/R injury; intracerebroventricular injection （icy） of losartan, an Ang Ⅱ AT1 receptor antagonist, could eliminate the protective effect of Ang Ⅱ against the I/R - induced gastric mucosal injury. ② NF -κB （ p65 ） was mainly expressed in the cytoplasm in the middle and bottom layers of gastric mucosa in normal rat, NF -κB was obviously expressed in both nucleus and cytoplasm and translocated to the nuclei of gastric mucosal cells after 1 h of L/R. Microinjection of Ang Ⅱ （30 ng） into the PVN significantly suppressed the translocation of p65 to the nuclei, and losartan blocked the effect of Ang Ⅱ. The expression of NF -κB varied with the changes of the gastric mucosal damage index. Conclusion Microinjection of AngⅡ into the PVN protects against GI/R injury, and the protective effect of Ang Ⅱ can be reversed by losartan. NF-κB may play an important role in the PVN, where Ang Ⅱ mediates the protection against GI/R injury .

关 键 词：室旁核 胃缺血/再灌注 血管紧张素Ⅱ 核因子ΚB 

分 类 号：R573.39[医药卫生—消化系统]