制备条件对磷脂酰胆碱单分子膜相变行为的影响  

Influence of the Preparation Methods to the Phases Transformations of Single-layer Phosphatidylcholine Film

作　　者：朱杰[1] 郭连红[1] 胡静[2] 王国栋[1] 

机构地区：[1]西北农林科技大学理学院,杨凌712100 [2]陕西师范大学物理学院,西安710062

出　　处：《生命科学仪器》2007年第12期15-23,共9页Life Science Instruments

基　　金：西北农林科技大学人才基金项目(编号:01140501);国家自然科学基金项目(编号:20572067)

摘　　要：目的:全面探讨制备条件对LB膜相变行为的影响可获得试验室特定设备及条件下制备磷脂LB膜的最佳条件。方法:用热力学相变理论类比描述了单分子膜的相变过程理论,并通过该理论,以π-A曲线和相变过程为主要分析点,系统地研究了磷脂铺展量、铺展浓度、铺展溶剂、铺展时间、压膜速度、亚相温度、亚相pH值等七大因素对成膜质量的影响。结果:磷脂铺展量、亚相的温度和pH值对磷脂单分子膜的质量影响最大,铺展浓度、铺展溶剂、铺展时间的影响要小得多,而在一定范围内压膜速度的大小基本上不影响膜的质量。结论:50μL,0.67mmol/ml的磷脂/乙醚溶液在pH6.8的亚相上铺展20min并以5mm/min的速度挤压单分子膜可获得完整的π-A曲线。Aims： Biologic simulated membranes in high stability and integrality are propitious to the advancement of the repetitionneeding experiments in biology. Deep discussion on the influence of the preparation methods to the phase transformations of single molecular Phosphatidylcholine film could reach the optimum parameters in special laboratory. Methods： Based on the analysis results of the π-A curves of Phosphatidylcholine film and the theoretic on the phase transformations, we study on the main seven factors or parameters by the numbers to the quality of the LB film such as inserting quantity and concentration of Phosphatidylcholine molecules, the solvents, expanding time, the compression velocity of the barriers, the temperature and pH value of the subphase and so forth. Results： Experimental results from the seven different conditions indicate that the influence of inserting quantity, the temperature and pH value of the subphase are biggest, and the concentration of Phosphatidylcholine molecules, the solvents and the expanding time are smaller, and the compression velocity of the barriers smallest. Conclusion： According to the analysis combined with the feasibility in special laboratory environments, we reach to the best parameters in film preparations： 50 μL, 0.67mmol/ml Phosphatidylcholine / ether on the subphase in pH6.8 expanded 20min can get the intact π-A curves when the film is compressed by barriers in 5mm/min.

关 键 词：磷脂酰胆碱 单分子膜 LB膜技术 π-A曲线 成膜条件 

分 类 号：Q554.1[生物学—生物化学]