咖啡因对胰α-淀粉酶部分理化性质的影响  

The Partial Influence of Caffeine on Pancreatic Amylase

作　　者：郑芸[1] 雷芳[1] 刘芯韵[1] 刘雯[1] 刘鑫[1] 李佳[1] 刘克武[1] 

机构地区：[1]四川大学生命学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《天然产物研究与开发》2007年第6期1016-1019,共4页Natural Product Research and Development

摘　　要：运用紫外光谱和荧光光谱技术研究咖啡因与胰α-淀粉酶的结合。反应类型为非竞争性抑制。Ki值为0.47×10-2mol/L。根据Stern-Volmer方程,计算了咖啡因对胰α-淀粉酶的表观淬灭常数,推测其荧光影响可能属于静态淬灭机制。By using ultraviolet and fluorescent spectrometry, we study the relation between caffeine and pancreatic amylase. The reaction in this research was investigated. The Ki was 0.47 × 10^-2 mol/L. According to the Stern-Volmer equations ,we compute the expressing quench parameter of pancreatic amylase influenced by caffeine. We guessed that the underlying mechanism belong to static quench.

关 键 词：胰α-淀粉酶 咖啡因 抑制作用 紫外光谱 荧光光谱 

分 类 号：Q556.9[生物学—生物化学]