毛细管电泳分离脑脊液中神经递质类氨基酸及其临床应用  

Separation of Neurotransmitter Amino Acids by Capillary Electrophoresis with Laser-induced Fluorescence in Cerebrospinal Fluid and its Clinical Application

作　　者：王跃国[1] 黄荣[1] 王惠民[1] 鞠少卿[1] 杨春兰[1] 黄一红[1] 王忠慧[1] 

机构地区：[1]南通大学附属医院检验科,江苏南通226001

出　　处：《现代检验医学杂志》2007年第6期5-8,共4页Journal of Modern Laboratory Medicine

基　　金：江苏省南通市社会发展基金资助课题(S2006030)

摘　　要：目的建立快速测定人脑脊液中神经递质类氨基酸的方法。方法用高效毛细管电泳-激光诱导荧光法检测了40例正常脑脊液、38例病毒性脑炎病人脑脊液、42倒脑出血病人脑脊液神经递质类氨基酸。结果在20rain内分离出4种氨基酸，线性范围为0．1～50μmol／L，峰的迁移时间RSD0．56％～2．06％，峰面积RSD2．24％～3．79％；回收率为98％以上。结论高效毛细管电泳-激光诱导荧光法具有快速、准确、灵敏的特点，可作为临床检测脑脊液标本中的神经递质类氨基酸的方法。Objective To establish a quick determination method of neurotransmitter amino acids of clinical patient cerebrospinal fluid （CSF）. Methods 120 patient CSF samples were detected by capillary electrophorsis with laser-induced fluorescence. Results 4 neurotransmitter amino acids were separated on base line in 20 min. Linear range of calibration curves was during 0. 1-50 μmol/L concentration,relative standard deviation （RSD） of peak time of mobility was 0. 56% -2.06% ,that of peak area 2.24%-3.79%. The recovery for 4 amino acid exceeded 98%. Conclusion Capillary electrophoresis with laser-induced fluorescence can be useful as a selective ,rapid and sensitive tool for study of the mechanisms of these disorders.

关 键 词：氨基酸 毛细管电泳-激光诱导荧光 荧光素异硫氰酸酯(FITC) 

分 类 号：Q503[生物学—生物化学] R446.14[医药卫生—诊断学]