一氧化氮在内毒素致细胞损伤过程中的变化及意义  

Changes of nitric oxide during cellular damage by endotoxin and its significance

作　　者：杨自力 王正国[1,2] 朱佩芳[1,2] 白涛 吴恒[1,2] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所第四研究室 [2]广州军区总医院危重病研究所

出　　处：《第三军医大学学报》1997年第3期202-204,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金;广东自然科学基金

摘　　要：目的：观察内毒素损伤内皮细胞过程中一氧化氮（ＮＯ）的变化及其意义。方法：选择体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为研究对象，分别用内毒素及硝基左旋精氨酸（ＬＮＮＡ）对之进行处理，并检测其上清液中ＮＯ－２／ＮＯ－３丙二醛（ＭＤＡ）含量及乳酸脱氢酶（ＬＤＨ）活性变化。结果：ＬＮＮＡ可使内皮细胞ＮＯ合成降低，ＭＤＡ含量和ＬＤＨ活性下降，而ＬＰＳ组上述各项指标则明显上升。结论：内毒素可导致内皮细胞，ＮＯ增多并与氧自由基相互作用，从而引发内皮细胞损伤。Objective: The changes of nitric oxide (NO) during cellular damage by endotoxin and its significance were studied. Methods: After the cultured human umbilical vein endothelial cells (HUVECs) were handled with endotoxin and LNNA respectively, the contents of NO-2/NO-3 and MDA and the changes of LDH activity in its supernatant were determined. Results: LNNA could inhibit the synthesis of NO and decrease the content of MDA and LDH activity. Conclusion: The overproduction of NO induced by endotoxin and the interaction between NO and oxygen free radicals might account for the damage of endothelial cells.

关 键 词：内毒素 内皮细胞 一氧化氮 细胞损伤 

分 类 号：R361.3[医药卫生—病理学] R631[医药卫生—基础医学]